Summary
In batch culture on reiinforced clostridial medium strain-dependent product profiles from glycerol revealed unusual fermentation products such as propionate and n-propanol with Clostridium butyricum LMG 1213t1, and 1,3-propanediol with C. butyricum LMG 1212t2 and C. pasteurianium LMG 3285. Only the latter two strains were able to grow on glycerol in a minimal medium. Nicotinamide adenine dinucleotide (NAD+)-dependent dehydrogenase activities were detected with 1,3-propanediol and n-butanol as substrate (the latter only after a lag period) in cell-free extracts of C. butyricum LMG 1212t2 and with 1,3-propanediol, n-butanol and ethanol in cell-free extracts of C. pasteurianum LMG 3285. The data indicated the existance of a specific 1,3-propanediol dehydrogenase in both organisms. In a chemostat, C. butyricum LMG 1212t2 converted 65% of the glycerol supplied as sole carbon and energy source to 1,3-propanediol without H2 production. Increasing concentration of acetate in the inflow medium resulted in less 1,3-propanediol and more butyrate and H2 production. C. pasteurianum LMG 3285 converted somewhat more than half of the glycerol supplied as sole energy and carbon source to n-butanol with significant concomitant H2 production. This fermentation pattern was hardly affected by acetate as co-substrate.
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References
Abeles RH, Brownstein AM, Randles CH (1960) β-Hydroxypropionaldehyde, an intermediate in the formation of 1,3-propanediol by Aerobacter aerogenes. Biochim Biophys Acta 41:530–531
Dams E, Huysmans E, Vandeberghe A, De Wachter R (1987) Structure of clostridial 5S ribosomal RNAs and bacterial evolution. Syst Appl Microbiol 9:54–61
Davis BJ (1964) Disc electrophoresis. II. Method and application to human serum proteins. Ann NY Acad Sci 121:404–427
De Vos P, Stevens P, De Ley J (1983) Hydrogen gas production from formate and glucose by different members of the Enterobacteriaceae. Biotechnol Lett 5:69–74
Forage RG, Foster MA (1982) Glycerol fermentation in Klebsiella pneumoniae: functions of the coenzyme B12-dependent glycerol and diol dehydratases. J Bacteriol 149:413–419
Forsberg CW (1987) Production of 1,3-propanediol from glycerol by Clostridium acetobutylicum and other Clostridium species. Appl Environ Microbiol 53:639–643
Heyndrickx M, Vansteenbeeck A, De Vos P, De Ley J (1986) Hydrogen gas production from continuous fermentation of glucose in a minimal medium with Clostridium butyricum LMG 1213t1. Syst Appl Microbiol 8:239–244
Heyndrickx M, De Vos P, Thibau B, Stevens P, De Ley J (1987) Effect of various external factors on the fermentative production of hydrogen gas from glucose by Clostridium butyricum strains in batch culture. Syst Appl Microbiol 9:163–168
Heyndrickx M, De Vos P, Speybrouck A, De Ley J (1989) Fermentation of mannitol by Clostridium butyricum: role of acetate as an external hydrogen acceptor. Appl Microbiol Biotechnol 31:323–328
Heyndrickx M, De Vos P, De Ley J (1991) Fermentation characteristics of Clostridium pasteurianum LMG 3285 grown on glucose and mannitol. J Appl Bacteriol In press
Johnson JL, Francis BS (1975) Taxonomy of the clostridia: ribosomal ribonucleic acid homologies among the species. J Gen Microbiol 88:229–244
Johnson EA, Lin ECC (1987) Klebsiella pneumoniae 1,3-propanediol: NAD+ oxidoreductase. J Bacteriol 169:2050–2054
Jungermann K, Thauer RK, Leimenstoll G, Decker K (1973) Function of reduced pyridine nucleotide-ferredoxin oxidoreductases in saccharolytic clostridia. Biochim Biophys Acta 305:268–280
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Nakas JP, Schaedle M, Parkinson CM, Coonley CE, Tanenbaum SW (1983) System development for linked-fermentation production of solvents from algal biomass. Appl Environ Microbiol 46:1017–1023
Petitdemange H, Cherrier C, Raval G, Gay R (1976) Regulation of the NADH and NADPH-ferredoxin oxidoreductases in clostridia of the butyric group. Biochim Biophys Acta 421:334–347
Rao G, Mutharasan R (1989) NADH levels and solventogenesis in Clostridium acetobutylicum: new insights through culture fluorescence. Appl Microbiol Biotechnol 30:59–66
Raspoet D, Pot B, De Deyn D, De Vos P, Kersters K, De Ley J (1991) Differentiation between 2,3-butanediol producing Bacillus licheniformis and B. polymyxa strains by fermentation product profiles and whole-cell protein electrophoretic patterns. Syst Appl Microbiol 13:1–7
Schütz H, Radler F (1984) Anaerobic reduction of glycerol to propanediol-1,3 by Lactobacillus brevis and Lactobacillus buchneri. Syst Appl Microbiol 5:169–178
Toraya T, Kuno S, Fukui S (1980) Distribution of coenzyme B12-dependent diol dehydratase and glycerol dehydratase in selected genera of Enterobacteriaceae and Propionibacteriaceae. J Bacteriol 141:1439–1442
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Heyndrickx, M., De Vos, P., Vancanneyt, M. et al. The fermentation of glycerol byClostridium butyricum LMG 1212t2 and 1213t1 andC. pasteurianum LMG 3285. Appl Microbiol Biotechnol 34, 637–642 (1991). https://doi.org/10.1007/BF00167914
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DOI: https://doi.org/10.1007/BF00167914