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Sclerotization as a long-term preservation method for Rosellinia necatrix strains

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Mycoscience

Abstract

This work describes a simple protocol for long-term preservation of strains of Rosellinia necatrix based on sclerotia production combined with storage at 4°C in liquid substrate, without affecting the growth and pathogenic characteristics of the fungal isolates recovered. The sclerotization process was set up in both liquid and solid media, and the sclerotia-like structures (pseudosclerotia) obtained were preserved in liquid media or water at 4°C. R. necatrix pseudosclerotia viability after 6 years of preservation at 4°C was confirmed by growth and microscopic characteristics, with no differences when compared with the fungal strains routinely preserved by periodic transfers. Additionally, pathogenicity on avocado plants by the preserved R. necatrix strains showed no difference from those preserved by periodic transfers. The albino strain used in this study should continue to be preserved by periodic subculturing.

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Acknowledgments

We thank Dr. Carlos López-Herrera for the generous supply of Rosellina necatrix strains from his personal collection. We gratefully acknowledge funding for the AGL08-05453-C02-01 and AGL11-30545-C02-01 projects from the Plan Nacional de I+D+I, Ministerio de Ciencia e Innovación, co-financed with FEDER (EU). C.E.C. was supported by a FPI Ph.D. fellowship from MCI. N.B. was supported by a FPU Ph.D. fellowship from MCI. We especially thank technician Irene Linares for construction and maintenance of the current collection of Rosellinia necatrix at the University of Málaga, Spain.

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Authors and Affiliations

  1. Departamento de Microbiología, Facultad de Ciencias, Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora,” IHSM-UMA-CSIC, Universidad de Málaga, Campus Universitario de Teatinos s/n, 29071, Málaga, Spain

    Jose A. Gutiérrez-Barranquero, Clara Pliego, Nuria Bonilla, Claudia E. Calderón, Alejandro Pérez-García, Antonio de Vicente & Francisco M. Cazorla

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  1. Jose A. Gutiérrez-Barranquero
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  2. Clara Pliego
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  3. Nuria Bonilla
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  4. Claudia E. Calderón
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  5. Alejandro Pérez-García
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  6. Antonio de Vicente
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  7. Francisco M. Cazorla
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Correspondence to Francisco M. Cazorla.

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Gutiérrez-Barranquero, J.A., Pliego, C., Bonilla, N. et al. Sclerotization as a long-term preservation method for Rosellinia necatrix strains. Mycoscience 53, 460–465 (2012). https://doi.org/10.1007/s10267-012-0185-0

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