Skip to main content
SpringerLink
Log in

Molecular detection and genetic analysis of porcine bocavirus in Korean domestic swine herds

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Several porcine bocaviruses have been detected worldwide, and this report is the first to describe this virus in a Korean domestic swine herd. We identified porcine bocavirus in various samples, including serum, tissue, feces and saliva, which revealed that porcine bocavirus predominates in Korean domestic swine populations. The results of this study also suggested that porcine bocaviruses primarily infect weaned piglets. Phylogenetic analysis of the ORF3 gene was performed to determine the genetic relationship of the Korean strains to reference strains from other countries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Blomström AL, Belak S, Fossum C, McKillen J, Allan G, Wallgren P, Berg M (2009) Detection of a novel porcine boca-like virus in the background of porcine circovirus type 2 induced postweaning multisystemic wasting syndrome. Virus Res 146:125–129

    Article  PubMed  Google Scholar 

  2. Blomström AL, Stahl K, Okurut AR, Masembe C, Berg M (2012) Genetic characterisation of a porcine bocavirus detected in domestic pigs in Uganda. Virus Genes 47(2):370–373

    Article  PubMed Central  PubMed  Google Scholar 

  3. Brieu N, Guyon G, Rodiere M, Segondy M, Foulongne V (2008) Human bocavirus infection in children with respiratory tract disease. Pediatr Infect Dis J 27(11):969–973

    Article  PubMed  Google Scholar 

  4. Cadar D, Cságola A, Lorincz M, Tombacz K, Kiss T, Spinu M, Tuboly T (2011) Genetic detection and analysis of porcine bocavirus type 1 (PoBoV1) in European wild boar (Sus scrofa). Virus Genes 43:376–379

    Article  CAS  PubMed  Google Scholar 

  5. Cheung AK, Wu G, Wang D, Bayles DO, Lager KM, Vincent AL (2010) Identification and molecular cloning of a novel porcine parvovirus. Arch Virol 155:801–806

    Article  CAS  PubMed  Google Scholar 

  6. Cságola A, Lorincz M, Cadar D, Tombacz K, Biksi I, Tuboly T (2012) Detection, prevalence and analysis of emerging porcine parvovirus infections. Arch Virol 157:1003–1010

    Article  PubMed  Google Scholar 

  7. Durham PJ, Lax A, Johnson RH (1985) Pathological and virological studies of experimental parvoviral enteritis in calves. Res Vet Sci 38:209–219

    CAS  PubMed  Google Scholar 

  8. Li B, Xiao S, Ma J, Liu Y, Mao L, Wen L, Mao A, Zhang X, Ni Y, Guo R, Zhou J, Yu Z, Lv L, Wang X, Fang L, Chen H, He K (2011) Development of a novel TaqMan-based real-time PCR assay for the detection of porcine boca-like virus (Pbo-likeV). Virol J 8:357

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Li B, Ma J, Xiao S, Fang L, Zeng S, Wen L, Zhang X, Ni Y, Guo R, Yu Z, Zhou J, Mao A, Lv L, Wang X, He K (2012) Complete genome sequence of a novel species of porcine bocavirus, PBoV5. J Virol 86:1286–1287

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Martin ET, Taylor J, Kuypers J, Magaret A, Wald A, Zerr D, Englund JA (2009) Detection of bocavirus in saliva of children with and without respiratory illness. J Clin Microbiol 47:4131–4132

    Article  PubMed Central  PubMed  Google Scholar 

  11. Sun B, Cai Y, Li Y, Li J, Liu K, Li Y, Yang Y (2013) The nonstructural protein NP1 of human bocavirus 1 induces cell cycle arrest and apoptosis in Hela cells. Virology 440:75–83

    Article  CAS  PubMed  Google Scholar 

  12. Sun Y, Chen AY, Cheng F, Guan W, Johnson FB, Qiu J (2009) Molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses. J Virol 83:3956–3967

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  13. Xiao CT, Halbur PG, Opriessnig T (2013) Molecular evolutionary genetic analysis of emerging parvoviruses identified in pigs. Infect Genet Evol 16:369–376

    Article  CAS  PubMed  Google Scholar 

  14. Yang WZ, Yu JM, Li JS, Cheng WX, Huang CP, Duan ZJ (2012) Genome characterization of a novel porcine bocavirus. Arch Virol 157:2125–2132

    Article  CAS  PubMed  Google Scholar 

  15. Zeng S, Wang D, Fang L, Ma J, Song T, Zhang R, Chen H, Xiao S (2011) Complete coding sequences and phylogenetic analysis of porcine bocavirus. J Gen Virol 92:784–788

    Article  CAS  PubMed  Google Scholar 

  16. Zhai S, Yue C, Wei Z, Long J, Ran D, Lin T, Deng Y, Huang L, Sun L, Zheng H, Gao F, Zheng H, Chen S, Yuan S (2010) High prevalence of a novel porcine bocavirus in weanling piglets with respiratory tract symptoms in China. Arch Virol 155:1313–1317

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgement

This study was supported by a grant (PJ009015) from BioGreen 21 Program, Republic of Korea.

Author information

Authors and Affiliations

  1. Department of Veterinary Medicine Virology Laboratory, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, Korea

    Min-Gyung Choi, Van-Giap Nguyen, Hee-Chun Chung, A-Reum Kim & Bong-Kyun Park

  2. Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea

    Seong-Jun Park

  3. Department of Microbiology and Infectious diseases, Faculty of Veterinary Medicine, Hanoi University of Agriculture, Hanoi, Vietnam

    Van-Giap Nguyen

Authors
  1. Min-Gyung Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seong-Jun Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Van-Giap Nguyen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hee-Chun Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A-Reum Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bong-Kyun Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bong-Kyun Park.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 18 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Choi, MG., Park, SJ., Nguyen, VG. et al. Molecular detection and genetic analysis of porcine bocavirus in Korean domestic swine herds. Arch Virol 159, 1487–1492 (2014). https://doi.org/10.1007/s00705-013-1944-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-013-1944-8

Keywords

Search

Navigation