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Application of polypyrrole/GOx film to glucose biosensor based on electrochemical-surface plasmon resonance technique

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Abstract

A surface plasmon resonance (SPR) based biosensor for glucose is presented in which a thin gold film modified with polypyrrole and glucose oxidase (PPy-GOx) acts as the sensor chip. It is based on SPR response to the change of refractive index of PPy-GOx film by the enzymatic catalytic reaction. The co-electropolymerization of pyrrole and GOx was carried out under cyclic voltammetric conditions, and simultaneously monitored by in-situ SPR. It has been revealed that the enzymatic reaction between GOx and PPy in the presence of glucose can lead to distinct changes in the SPR signal. From the experiments, a linear relationship was obtained in the range 1–100 μmol L−1 between glucose concentration and the rate of redox transformation of PPy. The detection limit was 0.5 μmol L−1 (S/N = 3) and recoveries were 95.2–102.7%.

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Acknowledgements

The study was supported by grant of Innovation Foundation of Xiamen University ( XDKJCX20053011) and Changchun Normal University Natural Science Foundation.

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Authors and Affiliations

  1. College of Chemistry, Changchun Normal University, Changchun, 130032, China

    Li Tian

  2. State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Department of Chemistry, Xiamen University, Xiamen, Fujian, 361005, China

    Jin Qiu, You-Chen Zhou & Shi-Gang Sun

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  1. Li Tian
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  2. Jin Qiu
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  3. You-Chen Zhou
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  4. Shi-Gang Sun
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Correspondence to Li Tian.

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Tian, L., Qiu, J., Zhou, YC. et al. Application of polypyrrole/GOx film to glucose biosensor based on electrochemical-surface plasmon resonance technique. Microchim Acta 169, 269–275 (2010). https://doi.org/10.1007/s00604-010-0344-y

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  • DOI: https://doi.org/10.1007/s00604-010-0344-y

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