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Differential diagnosis and molecular characterization of Hymenolepis nana and Hymenolepis diminuta (Cestoda: Cyclophyllidea: Hymenolepididae) based on nuclear rDNA ITS2 gene marker

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Abstract

Given the widespread distribution and medical implication of members of the genus Hymenolepis, specific identification of the aetiological agent becomes imperative. For precise diagnosis of the species, molecular techniques such as PCR and RFLP of the nuclear ribosomal internal transcribed spacer 2 (rDNA-ITS2) gene marker were carried out. The results showed distinct restriction patterns for both Hymenolepis nana and Hymenolepis diminuta when digested with either of the enzymes RsaI, HaeIII or HhaI. The annotated rDNA-ITS2 sequences from the two species revealed differences in the length; the folded secondary structure also depicted clear demarcation between the two species with variations in length of the helices, pyrimidine-pyrimidine mismatches and sites where motifs occur. In phylogenetic analysis of the evolutionary relationship between the two species as well as with other members of the family Hymenolepididae, the species causing human hymenolepiasis were found to be distantly related as they diverged independently from the ancestral lineage.

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Acknowledgments

The study was supported by the Indian Council of Medical Research (ICMR)-sponsored project ‘Molecular characterization of food-borne trematodes and cestodes prevailing in Northeast India’, sanctioned to V.T. and B.R. et al. The authors thankfully acknowledge the Departmental Special Assistance (DSA)-University Grants Commission-Special Assistance Programme (UGC-SAP) for infrastructural support to the Department of Zoology, NEHU, Shillong. We thank the Bioinformatics Centre (BIC), NEHU for allowing access to its facility and UGC for financial support as fellowship to SS.

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  1. Department of Zoology, North-Eastern Hill University, Shillong, 793022, Meghalaya, India

    Sunil Sharma, Damanbha Lyngdoh, Bishnupada Roy & Veena Tandon

  2. Biotech Park, Lucknow, 226021, Uttar Pradesh, India

    Veena Tandon

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  1. Sunil Sharma
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  2. Damanbha Lyngdoh
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  4. Veena Tandon
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Correspondence to Veena Tandon.

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Sharma, S., Lyngdoh, D., Roy, B. et al. Differential diagnosis and molecular characterization of Hymenolepis nana and Hymenolepis diminuta (Cestoda: Cyclophyllidea: Hymenolepididae) based on nuclear rDNA ITS2 gene marker. Parasitol Res 115, 4293–4298 (2016). https://doi.org/10.1007/s00436-016-5210-3

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