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Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry

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Abstract

Ascaris lumbricoides is responsible for a highly disseminated helminth parasitic disease, ascariosis, a relevant parasitosis that responds for great financial burden on the public health system of developing countries. In this work, metabolic fingerprinting using high-resolution mass spectrometry (HRMS) was employed to identify marker molecules from A. lumbricoides in different development stages. We have identified nine biomarkers, such as pheromones and steroidal prohormones in early stages, among other molecules in late development stages, making up four molecules for fertilized eggs, four marker molecules for first larvae (L1) and one marker molecule for third larvae (L3). Therefore, our findings indicate that this approach is suitable for biochemical characterization of A. lumbricoides development stages. Moreover, the straightforward analytical method employed, with almost no sample preparation from a complex matrix (feces) using high-resolution mass spectrometry, suggests that it is possible to seek for an easier and faster way to study animal molding processes.

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Acknowledgments

INNOVARE Biomarkers Laboratory would like to thank the Coordination for the Improvement of Higher Level (CAPES), the University of Campinas (Unicamp) and São Paulo Research Foundation (FAPESP, Process Nos. 11/50400-0, 14/00084-2, 14/00302-0 and 15/06809-1).

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Authors and Affiliations

  1. Innovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, São Paulo, Brazil

    Carlos Fernando Odir Rodrigues Melo, Cibele Zanardi Esteves, Tatiane Melina Guerreiro, Diogo Noin de Oliveira, Estela de Oliveira Lima & Rodrigo Ramos Catharino

  2. Biology Institute, Animal Biology Department, University of Campinas, Campinas, São Paulo, Brazil

    Rosimeire Nunes de Oliveira & Silmara Marques Allegretti

  3. Department of Clinical and Toxicological Analysis, State University of Ponta Grossa- UEPG, Ponta Grossa, Paraná, Brazil

    Júlio César Miné

Authors
  1. Carlos Fernando Odir Rodrigues Melo
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  2. Cibele Zanardi Esteves
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  3. Rosimeire Nunes de Oliveira
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  4. Tatiane Melina Guerreiro
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  5. Diogo Noin de Oliveira
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  6. Estela de Oliveira Lima
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  7. Júlio César Miné
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  8. Silmara Marques Allegretti
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  9. Rodrigo Ramos Catharino
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Corresponding authors

Correspondence to Silmara Marques Allegretti or Rodrigo Ramos Catharino.

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The authors declare that they have no conflict of interest.

Research involving human participants

This study obtained the approval of the Ethics Review Board of the State University of Ponta Grossa (Protocol 501171), and all experiments were performed according to the Board’s guidelines.

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Informed consent was obtained from all individual participants included in the study.

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Melo, C.F.O.R., Esteves, C.Z., de Oliveira, R.N. et al. Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry. Parasitol Res 115, 4107–4114 (2016). https://doi.org/10.1007/s00436-016-5183-2

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  • DOI: https://doi.org/10.1007/s00436-016-5183-2

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