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Hematological alterations caused by high intensity of L3 larvae of Contracaecum sp Railliet & Henry, 1912 (Nematoda, Anisakidae) in the stomach of Hoplias malabaricus in lakes in Pirassununga, São Paulo

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Abstract

The objective of the present study was to discover the hematological indices of Hoplias malabaricus infected by larvae of Contracaecum sp. A total of 105 fish were collected from two lakes located in the municipal district of Pirassununga, São Paulo, Brazil, of which 95.2 % were infested by L3 larvae of Contracaecum sp., with an average intensity of 348.7 ± 231.55 helminths/fish. A total of 76 fish were analyzed to establish hematological parameters. Following analysis, it was found that there was a significant difference between hematological parameters Hct, erythrocytes (Er), thrombocytopenia (p = 0.01), and Hb (p = 0.02) in lakes A and B, while there was no significant difference for leukocyte (p = 0.68), mean corpuscular volume, mean corpuscular hemoglobin (p = 0.06), and mean corpuscular hemoglobin concentration (p = 0.47). The intensity of infection correlated negatively with Er (r s  = −0.42; p < 0.01) and positively for (r s  = 0.48; p < 0.01). The high intensity of infection by Contracaecum sp. in H. malabaricus did not affect the health of the fish despite causing alterations in hematological variables.

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Acknowledgments

The authors would like to thank Mr. Ricardo Afonso Torres de Oliveira, technician at ICMBio (Instituto Chico Mendes de Conservação da Biodiversidade), Dr. Maria de los Angeles Perez Lizama of Nupélia (Research Center for Limnology, Ichthyology and Aquaculture) for analysis and interpretation of the condition factor, and Prof. Dr. Arício Xavier Linhares for statistical analysis. The author is grateful to CAPES/Procad-NF 69/2010-UNICAMP for financial support.

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Authors and Affiliations

  1. Department of Animal Biology, IB, UNICAMP, Cidade Universitária “Zeferino Vaz”, CP 6109, CEP 13083-862, Campinas, SP, Brazil

    Marlene T. Ueta

  2. Ictioparasitology Laboratory, Research Center for Limnology, Ichthyology and Aquaculture, NUPÉLIA, Universidade Estadual de Maringá, G-90, Avenida Colombo, 5790, CEP 87020-900, Maringá, PR, Brazil

    Letícia C. Karling & Ricardo M. Takemoto

  3. CEPTA/ICMBio—Instituto Chico Mendes de Conservação da Biodiversidade, BR SP 201, Km 6.5, CP 64, CEP 13630-970, Pirassununga, SP, Brazil

    Paulo S. Ceccarelli

  4. Department of Parasitology, UNICAMP, Campinas, Brazil

    Lincoln L. Corrêa

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  1. Lincoln L. Corrêa
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  2. Letícia C. Karling
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Correspondence to Lincoln L. Corrêa.

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Lincoln. L. Corrêa is a student of Parasitology, UNICAMP.

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Corrêa, L.L., Karling, L.C., Takemoto, R.M. et al. Hematological alterations caused by high intensity of L3 larvae of Contracaecum sp Railliet & Henry, 1912 (Nematoda, Anisakidae) in the stomach of Hoplias malabaricus in lakes in Pirassununga, São Paulo. Parasitol Res 112, 2783–2789 (2013). https://doi.org/10.1007/s00436-013-3446-8

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