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Purification and cellular localization of the Entamoeba histolytica transcarboxylase

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Abstract

Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin–carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation.

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Acknowledgments

We thank Bruce Allan Larsen for reviewing the use of English in this manuscript and Teresita Cruz Hernández for technical support. This work was supported in part by grants from SEPI-IPN. A. Salas-Casas, A. Jarillo-Luna, L. Mendoza-Alcantar, E. Abarca-Rojano, and R. Campos-Rodríguez are fellows of COFAA and DEPI-IPN.

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Authors and Affiliations

  1. Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340, Mexico, D.F., Mexico

    E. Barbosa-Cabrera, A. Salas-Casas, S. Rojas-Hernández, E. Abarca-Rojano & R. Campos-Rodríguez

  2. Departamento de Morfología, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340, Mexico, D.F., Mexico

    A. Jarillo-Luna

  3. Departamento de Infectómica y Patogénesis Molecular del Centro de Investigación y Estudios Avanzados del IPN, A.P. 14-740, México, D.F., CP 07000, Mexico

    M. A. Rodríguez

Authors
  1. E. Barbosa-Cabrera
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  2. A. Salas-Casas
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  3. S. Rojas-Hernández
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  4. A. Jarillo-Luna
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  5. E. Abarca-Rojano
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  6. M. A. Rodríguez
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  7. R. Campos-Rodríguez
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Corresponding author

Correspondence to R. Campos-Rodríguez.

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Barbosa-Cabrera, E., Salas-Casas, A., Rojas-Hernández, S. et al. Purification and cellular localization of the Entamoeba histolytica transcarboxylase. Parasitol Res 111, 1401–1405 (2012). https://doi.org/10.1007/s00436-012-2898-6

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  • DOI: https://doi.org/10.1007/s00436-012-2898-6

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