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Parasagittal compartmentation of cerebellar mossy fibers as revealed by the patterned expression of vesicular glutamate transporters VGLUT1 and VGLUT2

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Abstract

The cerebellum receives sensory signals from spinocerebellar (lower limbs) and dorsal column nuclei (upper limbs) mossy fibers. In the cerebellum, mossy fibers terminate in bands that are topographically aligned with stripes of Purkinje cells. While much is known about the molecular heterogeneity of Purkinje cell stripes, little is known about whether mossy fiber compartments have distinct molecular profiles. Here, we show that the vesicular glutamate transporters VGLUT1 and VGLUT2, which mediate glutamate uptake into synaptic vesicles of excitatory neurons, are expressed in complementary bands of mossy fibers in the adult mouse cerebellum. Using a combination of immunohistochemistry and anterograde tracing, we found heavy VGLUT2 and weak VGLUT1 expression in bands of spinocerebellar mossy fibers. The adjacent bands, which are in part comprised of dorsal column nuclei mossy fibers, strongly express VGLUT1 and weakly express VGLUT2. Simultaneous injections of fluorescent tracers into the dorsal column nuclei and lower thoracic–upper lumbar spinal cord revealed that upper and lower limb sensory pathways innervate adjacent VGLUT1/VGLUT2 parasagittal bands. In summary, we demonstrate that VGLUT1 and VGLUT2 are differentially expressed by dorsal column nuclei and spinocerebellar mossy fibers, which project to complementary cerebellar bands and respect common compartmental boundaries in the adult mouse cerebellum.

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Acknowledgments

This work was supported by new investigator start-up funds from Albert Einstein College of Medicine of Yeshiva University to RVS.

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  1. Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, 10461, USA

    Samrawit A. Gebre, Stacey L. Reeber & Roy V. Sillitoe

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  1. Samrawit A. Gebre
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Correspondence to Roy V. Sillitoe.

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S. A. Gebre, S. L. Reeber contributed equally.

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429_2011_339_MOESM1_ESM.tif

Supplementary Fig. 1 VGLUT1 and VGLUT2 are expressed in heterogeneous mossy fiber domains in the hemispheres. a. VGLUT2-immunoreactive mossy fiber domains in Crus1 of mouse. b. Compared to VGLUT2, VGLUT1-immunoreactive mossy fiber terminals are more widely distributed in Crus1. c. VGLUT2 and VGLUT1 expression overlapped within a common domain. The arrows indicate a boundary between a subset of mossy fibers that are heavily immunoreactive for VGLUT1, but are only weakly reactive for VGLUT2. (TIFF 4,917 kb)

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Gebre, S.A., Reeber, S.L. & Sillitoe, R.V. Parasagittal compartmentation of cerebellar mossy fibers as revealed by the patterned expression of vesicular glutamate transporters VGLUT1 and VGLUT2. Brain Struct Funct 217, 165–180 (2012). https://doi.org/10.1007/s00429-011-0339-4

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