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Iron and reactive oxygen responses in Pinus sylvestris root cortical cells infected with different species of Heterobasidion annosum sensu lato

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Abstract

Defence mechanisms in trees are not well understood. We assessed whether distribution of iron ions and their co-localisation with reactive oxygen species in Pinus sylvestris root cells reflect differential preferences of the pathogens Heterobasidion annosum sensu stricto, H. parviporum and H. abietinum to the host. Strains of H. annosum s.s. characterised by a greater preference for P. sylvestris induced accumulation of superoxide (O2 −) in host cells 6 h after inoculation, whereas two peaks in accumulation of O2 − (after 4 and 48 h) were observed after infection with strains of the pathogens H. parviporum and H. abietinum, which have a lower preference for P. sylvestris. Moreover, strains of H. annosum s.s. caused increased production of hydrogen peroxide (H2O2) in P. sylvestris cells, in contrast with strains of the other two species (H. parviporum and H. abietinum). Following inoculation with H. annosum s.s. strains, H2O2 was correlated negatively with O2 − and correlated positively with ferrous iron (Fe2+). Co-localisation of Fe3+ with H2O2 may suggest that they are involved in inducing hypersensitive responses and eventually cell death in roots inoculated with H. annosum s.s. strains, in contrast with H. parviporum, in which other mechanisms operate when the host is parasitised.

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Abbreviations

HR:

Hypersensitive response

ROS:

Reactive oxygen species

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Acknowledgments

This research received financial support from the Polish Ministry of Science and Higher Education (Project No. NN 309 136935) and the Institute of Dendrology, Polish Academy of Sciences. We would like to thank Ludmiła Bladocha and Anna Blaszkowiak for excellent technical support. Two anonymous reviewers provided helpful comments.

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Authors and Affiliations

  1. Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035, Kórnik, Poland

    Joanna Mucha, Marzenna Guzicka & Marcin Zadworny

  2. Department of Forest Pathology, Faculty of Forestry, Poznan University of Life Sciences, 60-625, Poznan, Poland

    Piotr Łakomy

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  1. Joanna Mucha
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  2. Marzenna Guzicka
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Correspondence to Joanna Mucha.

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Mucha, J., Guzicka, M., Łakomy, P. et al. Iron and reactive oxygen responses in Pinus sylvestris root cortical cells infected with different species of Heterobasidion annosum sensu lato. Planta 236, 975–988 (2012). https://doi.org/10.1007/s00425-012-1646-6

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