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Effects of compost input and tillage intensity on soil microbial biomass and activity under Mediterranean conditions

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Abstract

Organic amendment and tillage reduction are two common practices to contrast soil organic matter decline, thus promoting sustainable cropping and carbon sequestration. In a horticultural land use system under Mediterranean climate, we evaluated the 9-year effects of two compost inputs (15 and 30 t ha−1 y−1, low and high input, respectively) and two tillage intensities (intensive and reduced) on soil macronutrients concentration, microbial biomass and activity. Total organic C, total N and POlsen were smaller in plots amended at low input, whilst intensive tillage decreased them at both compost inputs. These decreases in intensively tilled plots was ascribed to the disruption of soil aggregates, with consequent microbial degradation of the physically protected organic matter by oxidative processes. On the contrary, reduced tillage increased the extractable C, likely due to a higher protection of the most labile soil C fraction from the mineralization. Similarly, microbial biomass C and N increased following both doubling compost input and reducing tillage intensity, with a greater effect by the first factor. The higher values of cumulative 10-day basal and 20-h glucose-induced respiration, and metabolic quotient in intensively tilled plots suggested that high tillage favoured soil aggregate disruption and C accessibility. This was also confirmed by higher values of dehydrogenase activity/total organic C in those plots. Intensive tillage caused a higher soil aeration and organic substrates accessibility to microflora, thus undoing the fertility benefits provided by the high compost input. However, also a low compost input coupled to reduced tillage seemed to accomplish soil sustainability needs.

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References

  • Alexander M (1977) Introduction to soil microbiology, 2nd edn. Wiley, New York

    Google Scholar 

  • Alvarez R, Dìaz RA, Barbero N, Santanatoglia OJ, Blotta L (1995) Soil carbon, microbial biomass and CO2–C production from three tillage systems. Soil Tillage Res 33:17–28

    Article  Google Scholar 

  • Alvear M, Rosas A, Rouanet JL, Borie F (2005) Effects of three soil tillage systems on some biological activities in an Ultisol from southern Chile. Soil Tillage Res 82:195–202

    Article  Google Scholar 

  • Anderson JPE, Domsch KH (1978) A physiological method for the quantitative measurement of microbial biomass in soils. Soil Biol Biochem 10:215–221

    Article  CAS  Google Scholar 

  • Anderson JPE, Domsch KH (1989) Ratios of microbial biomass carbon to total carbon in arable soils. Soil Biol Biochem 21:471–479

    Article  Google Scholar 

  • Anderson JPE, Domsch KH (1990) Application of ecophysiological quotients (qCO2 and qD) on microbial biomasses from soil of different cropping histories. Soil Biol Biochem 22:251–255

    Article  Google Scholar 

  • Anderson TH, Domsch KH (1993) The metabolic quotient for CO2 (qCO2) a specific activity parameter to assess the effects of environmental conditions, such as pH, on the microbial biomass of forest soils. Soil Biol Biochem 25:393–395

    Article  Google Scholar 

  • Badalucco L, Rao M, Colombo C, Palumbo G, Laudicina VA, Gianfreda L (2010) Reversing agriculture from intensive to sustainable improves soil quality in a semiarid South Italian soil. Biol Fertil Soils 46:481–489

    Article  Google Scholar 

  • Balota EL, Filho AC, Andrade DS, Dick PR (2004) Long-term and crop rotation effects on microbial biomass and C and N mineralization in a Brazilian Oxisol. Soil Tillage Res 77:137–145

    Article  Google Scholar 

  • Beare MH, Hendrix PF, Coleman DC (1994) Water-stable aggregates and organic matter fractions in conventional and no-tillage soils. Soil Sci Soc Am J 58:777–786

    Article  Google Scholar 

  • Blagodatskaya E, Kuzyakov Y (2008) Mechanisms of real and apparent priming effects and their dependence on soil microbial biomass and community structure: critical review. Biol Fertil Soils 45:115–131

    Article  Google Scholar 

  • Bremmer JM (1996) Nitrogen—total. In: Sparks DL (ed) Methods of soil analysis. Part 3, Chemical Methods. SSSA Book Series No 5. SSSA and ASA, Madison, WI, pp 1085–1121

    Google Scholar 

  • Brookes PC, Landman A, Pruden G, Jenkinson DS (1985) Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biol Biochem 17:837–842

    Article  CAS  Google Scholar 

  • Chahal KS, Wagner GH (1965) Decomposition of organic matter in Sanborn field soils amended with 14C glucose. Soil Sci 100:96–103

    Article  CAS  Google Scholar 

  • Dalal RC (1982) Effect of plant growth and addition of plant residues on the phosphatase activity in soil. Plant Soil 66:265–269

    Article  CAS  Google Scholar 

  • De Luca TH (1998) Relationship of 0.5 M K2SO4 extractable anthrone-reactive carbon to indices of microbial activity on forest soils. Soil Biol Biochem 30:1293–1299

    Article  Google Scholar 

  • Dick RP, Rasmussen PE, Kerle EA (1988) Influence of long-term residue management on soil enzyme activities in relation to soil chemical properties of a wheat-fallow system. Biol Fertil Soils 6:159–164

    Article  CAS  Google Scholar 

  • Doran JW (1980) Soil microbial and biochemical changes associated with reduced tillage. Soil Sci Soc Am J 44:765–771

    Article  CAS  Google Scholar 

  • Eivazi F, Tabatabai MA (1977) Phosphatases in soils. Soil Biol Biochem 9:167–172

    Article  CAS  Google Scholar 

  • Eivazi F, Tabatabai MA (1988) Glucosidases and galactosidases in soils. Soil Biol Biochem 20:601–606

    Article  CAS  Google Scholar 

  • García C, Hernández T, Costa F (1994) Microbial activity in soils under Mediterranean environmental conditions. Soil Biol Biochem 26:1185–1191

    Article  Google Scholar 

  • García C, Hernández T, Costa F (1997) Potential use of dehydrogenase activity as an index of microbial activity in degraded soils. Commun Soil Sci Plant Anal 28:123–134

    Article  Google Scholar 

  • García-Ruiz R, Ochoa V, Hinojosa MB, Carreira JA (2008) Suitability of enzyme activities for the monitoring of soil quality improvement in organic agricultural systems. Soil Biol Biochem 40:2137–2145

    Article  Google Scholar 

  • Gupta VVSR, Germida JJ (1988) Distribution of microbial biomass and its activity in different soil aggregate size classes as affected by cultivation. Soil Biol Biochem 20:777–786

    Article  CAS  Google Scholar 

  • Hao XH, Liu SL, Wu JS, Hu RG, Tong CL, Su YY (2008) Effect of long-term application of inorganic fertilizer and organic amendments on soil organic matter and microbial biomass in three subtropical paddy soils. Nutr Cycl Agroecosyst 81:17–24

    Article  Google Scholar 

  • Insam H, Haselwandter K (1989) Metabolic quotient of the soil microflora in relation to plant succession. Oecologia 79:174–178

    Article  Google Scholar 

  • IUSS Working Group WRB (2006) World reference base for soil resources 2006, 2nd edn. World Soil Resources Reports 103. FAO, Rome

  • Kandeler E, Gerber H (1988) Short-term assay of soil urease activity using colorimetric determination of ammonium. Biol Fertil Soils 6:68–72

    Article  CAS  Google Scholar 

  • Kunito T, Saeki K, Goto S, Hayashi H, Oyaizu H, Matsumoto S (2001) Copper and zinc fractions affectingmicroorganisms in long term sludge-amended soils. Bioresour Technol 79:135–146

    Article  PubMed  CAS  Google Scholar 

  • Lagomarsino A, Moscatelli MC, Di Tizio A, Mancinelli R, Grego S, Marinari S (2009) Soil biochemical indicators as a tool to assess the short-term impact of agricultural management on changes in organic C in a Mediterranean environment. Ecol Indic 9:518–527

    Article  CAS  Google Scholar 

  • Laudicina VA, Hurtado MD, Badalucco L, Delgado A, Palazzolo E, Panno M (2009) Soil chemical and biochemical properties of a salt-marsh alluvial Spanish area after long-term reclamation. Biol Fertil Soil 45:691–700

    Article  CAS  Google Scholar 

  • Madejón E, Moreno F, Murillo JM, Pelegrìn F (2007) Soil biochemical response to long-term conservation tillage under semi-arid Mediterranean conditions. Soil Tillage Res 94:346–352

    Article  Google Scholar 

  • Madejón E, Murillo JM, Moreno F, López MV, Arrue JL, Alvaro-Fuentes J, Cantero R (2009) Effect of long-term conservation tillage on soil biochemical properties in Mediterranean Soanish area. Soil Tillage Res 105:55–62

    Article  Google Scholar 

  • Masciandaro G, Ceccanti B (1999) Assessing soil quality in different agro-ecosystems through biochemical and chemico-structural properties of humic substances. Soil Tillage Res 51:129–137

    Article  Google Scholar 

  • Melero S, Madejòn E, Ruiz JC, Herencia JF (2007) Chemical and biochemical properties of a clay soil under dryland agriculture system as affected by organic fertilization. Eur J Agron 26:327–334

    Article  CAS  Google Scholar 

  • Meli SM, Badalucco L, English LC, Hopkins DW (2003) Respiratory responses of soil micro-organisms to simple and complex organic substrates. Biol Fertil Soils 37:96–101

    CAS  Google Scholar 

  • Monokrousos N, Papatheodorou EM, Diamantopoulos JD, Stamou GP (2006) Soil quality variables in organically and conventionally cultivated field sites. Soil Biol Biochem 38:1282–1289

    Article  CAS  Google Scholar 

  • Nannipieri P, Ascher J, Ceccherini MT, Landi L, Pietramellara G, Renella G (2003) Microbial diversity and soil functions. Eur J Soil Sci 54:655–670

    Article  Google Scholar 

  • Nelson DW, Sommers LE (1996) Total carbon, organic carbon, and organic matter. In: Sparks DL (ed) Methods of Soil Analysis. Part 3, Chemical Methods. SSSA Book Series No 5. SSSA and ASA, Madison, WI, pp 961–1010

    Google Scholar 

  • Olsen SR, Cole CW, Watanabe FS, Dean LA (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate. U.S. Department of Agriculture, circular 939

  • Pankhurst CE, Kirkby CA, Hawke BG, Harch BD (2002) Impact of a change in tillage and crop residue management practice on soil chemical and microbiological properties in a cereal-producing red duplex soil in NSW, Australia. Biol Fertil Soils 35:189–196

    Article  CAS  Google Scholar 

  • Parkin TB, Doran JW, Franco-Vizcaìno E (1996) Field and laboratory tests of soil respiration. In: Doran JW, Jones AJ (eds) Methods for assessing soil quality, SSSA Special Publication 49. American Society of Agronomy, Madison, pp 231–245

    Google Scholar 

  • Peel MC, Finlayson BL, McMahon TA (2007) Updated world map of the Köppen-Geiger climate classification. Hydrol Earth Syst Sci 11:1633–1644

    Article  Google Scholar 

  • Prasad P, Basu S, Behra N (1994) A comparative account of microbiological characteristics of soils under natural forest, grassland and crop field from Eastern India. Plant Soil 175:85–91

    Article  Google Scholar 

  • Roldán A, Caravaca F, Hernández MT, Garcìa C, Sánchez-Brito C, Velásquez M, Tiscareño M (2003) No-tillage, crop residue additions, and legume cover cropping effects on soil quality characteristics under maize in Patzcuaro watershed (Mexico). Soil Tillage Res 72:65–73

    Article  Google Scholar 

  • Schjonning P, Elmholt S, Munkholm LJ, Debosz K (2002) Soil quality aspects of humid sandy loams as influenced by organic and conventional long-term management. Agric Ecosyst Environ 88:195–214

    Article  Google Scholar 

  • Skujins JJ (1967) Enzymes in soil. In: McLaren AD, Petersen GH (eds) Soil biochemistry, vol I. Marcel Dekker, New York, pp 371–414

    Google Scholar 

  • Smith JL, McNeal BL, Cheng HH (1985) Estimation of soil microbial biomass: an analysis of the respiratory response of soils. Soil Biol Biochem 17:11–16

    Article  CAS  Google Scholar 

  • Sumner ME, Miller WP (1996) Cation exchange capacity and exchange coefficients. In: Sparks DL (ed) Methods of soil analysis. Part 3, Chemical Methods. SSSA Book Series No 5. SSSA and ASA, Madison, WI, pp 1201–1229

    Google Scholar 

  • Tabatabai MA (1982) Soil Enzymes. In: Page AL (ed) Methods of soil analysis. Part 2, chemical and microbiological properties, 2nd edn. American Society of Agronomy, Madison, pp 903–943

    Google Scholar 

  • Tabatabai MA, Bremner JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307

    Article  CAS  Google Scholar 

  • Tabatabai MA, Bremner JM (1970) Arylsulphatase activity of soils. Soil Sci Soc Am Proc 34:427–429

    Article  CAS  Google Scholar 

  • Thalmann A (1968) Zur Methodik der Bestimmung der Dehydrogenaseaktivitat im Boden mittels Triphenyltetrazoliumchlorid (TTC). Landwirtsch Forsch 21:249–258

    CAS  Google Scholar 

  • Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19:703–707

    Article  CAS  Google Scholar 

  • Vidhana Arachchi LP (2009) Effect of deep ploughing on the water status of highly and less compacted soils for coconut (Cocos nucifera L.) production in Sri Lanka. Soil Tillage Res 103:350–355

    Article  Google Scholar 

  • Wardle DA, Ghani A (1995) A critique of the microbial quotient (qCO2) as a bio-indicator of disturbance and ecosystem development. Soil Biol Biochem 27:1601–1610

    Article  CAS  Google Scholar 

Download references

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  1. Dipartimento di Ingegneria e Tecnologie Agro-Forestali, University of Palermo, Viale delle Scienze, 13 (edificio 4), 90128, Palermo, Italy

    Vito Armando Laudicina, Luigi Badalucco & Eristanna Palazzolo

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  1. Vito Armando Laudicina
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  2. Luigi Badalucco
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  3. Eristanna Palazzolo
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Correspondence to Vito Armando Laudicina.

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Laudicina, V.A., Badalucco, L. & Palazzolo, E. Effects of compost input and tillage intensity on soil microbial biomass and activity under Mediterranean conditions. Biol Fertil Soils 47, 63–70 (2011). https://doi.org/10.1007/s00374-010-0502-8

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