Skip to main content
SpringerLink
Log in

White spot syndrome virus inactivation study by using gamma irradiation

  • Biology
  • Published:
Chinese Journal of Oceanology and Limnology Aims and scope Submit manuscript

Abstract

The present study was conducted to investigate the effect of gamma irradiation on white spot syndrome virus (WSSV). White spot syndrome virus is a pathogen of major economic importance in cultured penaeid shrimp industries. White spot disease can cause mortalities reaching 100% within 3–10 days of gross signs appearing. During the period of culture, immunostimulant agents and vaccines may provide potential methods to protect shrimps from opportunistic and pathogenic microrganisms. In this study, firstly, WSSV was isolated from infected shrimp and then multiplied in crayfish. WSSV was purified from the infected crayfish haemolymph by sucrose gradient and confirmed by transmission electron microscopy. In vivo virus titration was performed in shrimp, Penaeus semisulcatus. The LD50 of live virus stock was calculated 10 5.4/mL. Shrimp post-larvae (1–2 g) were treated with gamma-irradiated (different doses) WSSV (100 to 10−4 dilutions) for a period of 10 days. The dose/survival curve for irradiated and un-irradiated WSSV was drawn; the optimum dose range for inactivation of WSSV and unaltered antigenicity was obtained 14–15 kGy. This preliminary information suggests that shrimp appear to benefit from treatment with gammairradiated WSSV especially at 14–15 KGy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adams A. 1991. Response of penaeid shrimp to exposure to Vibrio species. Fish Shellfish Immunol., 1: 59–70.

    Article  Google Scholar 

  • Brown F. 2002. Inactivation of viruses by aziridines. Vaccine, 20: 322–327.

    Article  Google Scholar 

  • Cavalli L S, Marins L F, Netto S, Abreu P C. 2008. Evaluation of White Spot Syndrome Virus (WSSV) in wild shrimp after a major outbreak in shrimp farms at Laguna, southern Brazil. Atlântica Rio Grande, 30(1): 45–52.

    Google Scholar 

  • Chang P S, Lo C F, Wang Y C, Kou G H. 1996. Identification of white spot syndrome associated baculovirus (WSBV) target organs in the shrimp Penaeus monodon by in situ hybridization. Dis. Aquat. Organ., 27: 131–139.

    Article  Google Scholar 

  • Du H, Xu Z, Wu X, Li W, Dai W. 2006. Increased resistance to white spot syndrome virus in Procambarus clarkii by injection of envelope protein VP28 expressed using recombinant baculovirus. Aquaculture, 260: 39–43.

    Article  Google Scholar 

  • Escobedo-Bonilla C M, Wille M, Alday Sanz V, Sorgeloos Pensaert M B, Nauwynck J H. 2005. In vivo titration of white spot syndrome virus (WSSV) in specific pathogenfree Litopenaeus vannamei by intramuscular and oral routes. Dis. Aquat. Organ., 66: 163–170.

    Article  Google Scholar 

  • Ginoza W. 1968. Inactivation viruses by ionizing radiation and heat. In: Maramorosch K, Koprowski H eds. Methods in Virology, Vol.4. Academic Press, London, p.139–209.

    Google Scholar 

  • Heidarieh M, Afsharnasab M, Soltani M, Dashtyannasab A, Rajabifar S, Sheikhzadeh N, Tamimi A H. 2010. Effects of Ergosan and Vibromax to prevent vibriosis and WSSV in Litopenaeus vannamei. J. Fish. Aquat. Sci., 5: 120–125.

    Google Scholar 

  • Heidarieh M, Hedayatirad Maryam, MirvaghefiA R, Diallo A, Mousavi Sh, Sheikhzadeh N, Shahbazfar A A. 2014. Effect of gamma-irradiation on inactivation of Ichthyophthirius multifiliis trophonts and its efficacy on host response in experimentally immunized rainbow trout (Oncorhynchus mykiss). Turk. J. Vet. Anim. Sci., http://dx.doi.org/10.3906/vet-1312-78.

    Google Scholar 

  • Heidarieh M, Soltani M, Motamedi Sedeh F, Sheikhzadeh N. 2013. Low water temperature retards white spot syndrome virus replication in Astacus leptodactylus Crayfish. Acta Sci. Vet., 41: 1 135.

    Google Scholar 

  • Huang C H, Zhang L R, Zhang J H, Xiao L C, Wu Q J, Chen D H, Li J K K. 2001. Purification and characterization of white spot syndrome virus (WSSV) produced in an alternate host: crayfish, Cambarus clarkii. Virus Res., 76: 115–125.

    Article  Google Scholar 

  • Itami T, Takahashi Y, Nakamura Y. 1989. Efficacy of vaccination against vibriosis in cultured kuruma prawns Penaeus japonicus. J. Aquat. Anim. Health, 1: 234–242.

    Article  Google Scholar 

  • Karber G. 2002. Foot and mouth disease, Karber Formula for calculation of virus/antibody titres. OIE A Manual, Overview.

    Google Scholar 

  • Lombardo J H, Smolko E E. 1990. A Biotechnological project with a gamma radiation source of 100,000 Ci. Radiat. Phys. Chem., 35(4–6): 585–589.

    Google Scholar 

  • Motamedi Sedeh F, Afsharnasab M, Heidarieh M, Shafaee S K, Rajabifar S, Dashtiannasab A, Razavi M H. 2012. Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus. Iran. J. Fish. Sci., 11(1): 145–155.

    Google Scholar 

  • Motamedi Sedeh F, Khorasani A, Shafaee K, Fatolahi H, Arbabi K. 2008. Preparation of FMD type A87/IRN inactivated vaccine by gamma irradiation and the immune response on guinea pig. Indian J. Microbiol., 48(3): 326–330.

    Article  Google Scholar 

  • Motamedi Sedeh F, Soleimanjahi H, Jalilian A, Mahravani H. 2011. Inactivation of nano-FMDV type O/IRN/1/2007 particles infectivity using gamma irradiation. Indian J. Sci. Technol., 4(12): 1 654–1 656.

    Google Scholar 

  • Namikoshi A, Wu J L, Yamashita T, Nishizawa T, Nishioka T, Arimoto M, Muroga K. 2004. Vaccination trials with Penaeus japonicus to induce resistance to white spot syndrome virus. Aquaculture, 229: 25–35.

    Article  Google Scholar 

  • Ning J F, Zhu W, Xu J P, Zheng C Y, Meng X L. 2009. Oral delivery of DNA vaccine encoding VP28 against white spot syndrome virus in crayfish by attenuated Salmonella typhimurium. Vaccine, 27: 1 127–1 135.

    Article  Google Scholar 

  • Pollard E. 1955. The action of ionizing radiation on viruses. Advan. Virus Res., 2: 109–151.

    Article  Google Scholar 

  • Poulos B T, Pantoja C R, Bradley-Dunlop D, Aguilar J, Lightner D V. 2001. Development and application of monoclonal antibodies for the detection of white spot syndrome virus of Penaeid shrimp. Dis. Aquat. Organ., 47: 13–23.

    Article  Google Scholar 

  • Rajesh Kumar S, Ishaq Ahamed V P, Sarathi M, Nazeer Basha A, Sahul Hameed A S. 2008. Immunological responses of Penaeus monodon to DNA vaccine and its efficacy to protect shrimp against white spot syndrome virus (WSSV). Fish Shellfish Immunol., 24(4): 467–478.

    Article  Google Scholar 

  • Rowley A F, Powell A. 2007. Invertebrate immune systemsspecific, quasi-specific, or nonspecific? J. Immunol., 179: 7 209–7 214.

    Article  Google Scholar 

  • Smolko E E, Lombardo J H. 2005. Virus inactivation studies using ion beams, electron and gamma irradiation. Nucl. Instr. Meth. Phys. B, 236: 249–253.

    Article  Google Scholar 

  • Syaifudin M, Tetriana D, Darlina Nurhayati S. 2011. The feasibility of gamma irradiation for developing malaria vaccine. Atom Indones., 37(3): 91–101.

    Google Scholar 

  • Traifalgar R F M, Corre V L, Serrano A E. 2013. Efficacy of dietary immunostimulants to enhance the immunological responses and Vibriosis resistance of juvenile Penaeus monodon. J. Fish. Aquat. Sci., 8: 340–354.

    Google Scholar 

  • Tsai M F, Yu H T, Tzeng H F, Leu J H, Chou C M, Huang C J, Wang C H, Lin J Y, Kou G H, Lo C F. 2000. Identification and characterization of a shrimp white spot syndrome virus (WSSV) gene that encodes a novel chimeric polypeptide of cellular-type thymidine kinase and thymidylate kinase. Virolog, 277: 100–110.

    Article  Google Scholar 

  • van Hulten M C W, Tsai M F, Schipper C A, Lo C F, Kou G H, Valk J M. 2000. Analysis of a genomic segment of white spot syndrome virus of shrimp containing ribonucleotide reductase genes and repeat regions. J. Gen. Virol., 81: 307–316.

    Google Scholar 

  • van Hulten M C W, Witteveldt J, Snippe M, Valk J M. 2001. White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp. Virology, 285: 228–233.

    Article  Google Scholar 

  • Wang Y T, Liu W, Seah J N, Lam C S, Xiang J H, Korzh V, Kwang J. 2002. White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis. Dis. Aquat. Organ., 52: 249–259.

    Article  Google Scholar 

  • Witteveldt J, Cifuentes C C, Valk J M, Van Hulten M C W. 2004a. Protection of Penaeus monodo n against white spot syndrom virus by oral vaccination. J. Virol., 78(4): 2 057–2 061.

    Article  Google Scholar 

  • Witteveldt J, Valk J M, van Hulten MC W. 2004b. Protection of Penaeus monodon against white spot syndrome virus using a WSSV subunit vaccine. Fish Shellfish Immunol., 16: 571–579.

    Article  Google Scholar 

  • Wu J L, Nishioka T, Nishizaw T, Muroga K. 2002. A timecourse study on the resistance of Penaeus japonicus induced by artificial infection with white spot syndrome virus. Fish Shellfish Immunol., 13(5): 391–403.

    Article  Google Scholar 

  • Zhu F, Du H, Miao Z G, Quan H Z, Xu Z R. 2009. Protection of Procambarus clarkii against white spot syndrome virus using inactivated WSSV. Fish Shellfish Immunol., 26: 685–690.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Nuclear Science and Technology Research Institute, Karaj, Iran

    Marzieh Heidareh, Farahnaz Motamedi Sedeh & Saeed Rajabifar

  2. Veterinary Faculty, University of Tehran, Tehran, Iran

    Mehdi Soltani

  3. Iran Fisheries Research Institute, Tehran, Iran

    Mohammad Afsharnasab & Aghil Dashtiannasab

Authors
  1. Marzieh Heidareh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Farahnaz Motamedi Sedeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mehdi Soltani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Saeed Rajabifar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mohammad Afsharnasab
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Aghil Dashtiannasab
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Marzieh Heidareh or Farahnaz Motamedi Sedeh.

Additional information

Supported by the Nuclear Science and Technology Research Institute, Karaj, Iran (No. A87A061, 2009)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Heidareh, M., Sedeh, F.M., Soltani, M. et al. White spot syndrome virus inactivation study by using gamma irradiation. Chin. J. Ocean. Limnol. 32, 1024–1028 (2014). https://doi.org/10.1007/s00343-014-3333-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00343-014-3333-x

Keywords

Search

Navigation