Abstract
FMD is one of the most economically damaging diseases that affect livestock animals. In this study FMD Virus type A87/IRN was multiplied on BHK21 cells. The virus was titrated by TCID50 method, it was 107.5/ml. The FMD virus samples were inactivated by gamma ray from 60Co source at −20°C. Safety test was done by IBRS2 monolayer cell culture method, also antigenicity of irradiated and un-irradiated virus samples were studied by Complement Fixation Test. The Dose/Survival curve for irradiated FMD Virus was drawn, the optimum dose range for inactivation of FMDV type A87/IRN and unaltered antigenicity was obtained 40–44 kGy. The inactivated virus samples by irradiation and ethyleneimine (EI) were formulated respectively as vaccine with Al(OH)3 gel and other substances. The vaccines were inoculated to Guinea pigs and the results of Serum Neutralization Test for the normal vaccine and radio-vaccine showed protective titer after 8 months. The potency test of the inactivated vaccines was done, PD50 Value of the vaccines were calculated 7.06 and 5.6 for inactivated vaccine by EI and gamma irradiation respectively.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Zhidong Z and Soren A (2004) Quantitative analysis of foot and mouth disease virus RNA loads in bovine tissues. J Gene Virol 85:2567–2575
Barteling SJ and Vreeswijk J (1991) Developments in FMD vaccines. Vaccine 9:75–871
Pollard E (1955) The action of ionizing radiation on viruses Advan Virus Res 2:109–151
Dertinger H and Jung H (1970) The action of radiation on viruses. Molecular Radiation Biology Springer-Verlag, NY, pp 174–194
Lombardo JH and Smolko EE(1990) Biotechnological project with a gamma radiation source of 100,000 Ci. Radiat Phys Chem 35(4–6):585–589
Smolko EE and Lombardo JH (2005) Virus inactivation studies using ion beams, electron and gamma irradiation. Nuclear Instrument and Method in physics research B 236:249–253
Donn A, Castagnaro M and Donaldson AI (1995) Ultra structural and replicative of foot and mouth disease virus in persistently infected BHK-21 cells. Arch virol 140:13–25
Reed LJ and Muench H (1938) A simple method of estimating 50% end points. Am J Hyg 27:493–497
FRESCURA T and VIVOLI P (1973) Studies of the FMD virus sub-types using Antigens Inactivated by gamma radiations. Zbl Vet Med B 20:822–825
Kolmer JA (1928) Serum diagnosis by complement fixation test, Lea and Febiger Publishers, Philadelphia, 345 p
Balamurugan V, Renji R, Saha S N, Reddy G R and Gopalakishna S (2003) Protective immune response of the capsid precursor polypeptide of FMDV type O produced in Pichia Pastoris. Virus Rese 9:141–149
Berinstein A, Tami C, Taboga O, Smitsaart E and Carrillo E (2000) Protective immunity against foot and mouth disease virus induced by a recombinant vaccinia virus. Vaccine 18:2231–2238
Karber (2002) FMD, Kaber Formula for calculation of virus/antibody titres. OIEA Manual, Overview
Leucam F, Fedidia M and Dannacher G (1964) Measurement of post vaccinal immunity to foot and mouth disease in cattle by test on guinea pigs. Rev Med Vet 115:225–245
Pinto A A and Garland A J M (1979) Immune response to virus infection associated antigen in cattle repeated vaccinated with foot and mouth disease virus inactivated by formalin or acetylethyleneimine. J Hyg Camb 82:41–51
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sedeh, F.M., Khorasani, A., Shafaee, K. et al. Preparation of FMD type A87/IRN inactivated vaccine by gamma irradiation and the immune response on guinea pig. Indian J Microbiol 48, 326–330 (2008). https://doi.org/10.1007/s12088-008-0023-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12088-008-0023-4