Abstract
To further expand the application of anammox biotechnology, a novel zero-valent iron-assembled upflow anaerobic sludge bed reactor was employed to strengthen anammox performance under low temperature and shock load. Packed with sponge iron and polyester sponge, this novel reactor could speed up the recovery of anammox activity in 12 days and improve the adaptability of anammox bacteria at the temperature of 10–15 °C. The high nitrogen loading rate of 1109.2 mg N/L/day could be adapted in 27 days and the new nitrogen pathway under the effect of sponge iron was clarified by batch experiment. Moreover, the real-time quantitative PCR analysis and Illumina MiSeq sequencing verified the dominant status of Candidatus Kuenenia stuttgartiensis and planctomycete KSU-1, as well as demonstrated the positive role of sponge iron on anammox microorganisms’ proliferation. The findings might be beneficial to popularize anammox-related processes in municipal and industrial wastewater engineering.
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Acknowledgments
The authors gratefully acknowledge the support from the National Basic Research Program of China (no. 2013CB934301), National Natural Science Foundation of China (nos. 21477063 and 21177075), International S&T Cooperation Program of China (2013DFR30920), Tai Shan Scholar Foundation (no. ts201511003), Research Award Fund for Outstanding Middle-aged and Young Scientist of Shandong Province (no. BS2012HZ007 and BS2013SW038), Jinan Science and Technology Project (201401364), and Youth Scientific Research Foundation of Shandong Academy of Agricultural Sciences (2014QNM20).
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Long-Fei Ren and Lu Lv contributed equally to this work and share the first authorship.
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Ren, LF., Lv, L., Zhang, J. et al. Novel zero-valent iron-assembled reactor for strengthening anammox performance under low temperature. Appl Microbiol Biotechnol 100, 8711–8720 (2016). https://doi.org/10.1007/s00253-016-7586-5
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DOI: https://doi.org/10.1007/s00253-016-7586-5