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Comparative analyses of Podospora anserina secretomes reveal a large array of lignocellulose-active enzymes

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Abstract

The genome of the coprophilous fungus Podospora anserina harbors a large and highly diverse set of putative lignocellulose-acting enzymes. In this study, we investigated the enzymatic diversity of a broad range of P. anserina secretomes induced by various carbon sources (dextrin, glucose, xylose, arabinose, lactose, cellobiose, saccharose, Avicel, Solka-floc, birchwood xylan, wheat straw, maize bran, and sugar beet pulp (SBP)). Compared with the Trichoderma reesei enzymatic cocktail, P. anserina secretomes displayed similar cellulase, xylanase, and pectinase activities and greater arabinofuranosidase, arabinanase, and galactanase activities. The secretomes were further tested for their capacity to supplement a T. reesei cocktail. Four of them improved significantly the saccharification yield of steam-exploded wheat straw up to 48 %. Fine analysis of the P. anserina secretomes produced with Avicel and SBP using proteomics revealed a large array of CAZymes with a high number of GH6 and GH7 cellulases, CE1 esterases, GH43 arabinofuranosidases, and AA1 laccase-like multicopper oxidases. Moreover, a preponderance of AA9 (formerly GH61) was exclusively produced in the SBP condition. This study brings additional insights into the P. anserina enzymatic machinery and will facilitate the selection of promising targets for the development of future biorefineries.

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Acknowledgments

This study was funded by the French National Research Agency (ANR, program E-TRICEL ANR-07-BIOE-006). L. Poidevin received an Enerbio research grant from the ‘Tuck’Foundation.

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Author notes

  1. Laetitia Poidevin

    Present address: Department of Plant Pathology and Microbiology, University of California Riverside, Riverside, CA, USA

Authors and Affiliations

  1. Biotechnologie des Champignons Filamenteux, BCF, UMR 1163, INRA, Aix Marseille Université, Polytech, CP 925, 13228, Marseille Cedex 09, France

    Laetitia Poidevin, Jean-Guy Berrin, Chloé Bennati-Granier, Anthony Levasseur, Isabelle Herpoël-Gimbert & Eric Record

  2. IFP Energies Nouvelles, 1 et 4, Avenue de Bois-Préau, 92852, Rueil-Malmaison Cedex, France

    Laetitia Poidevin & Senta Heiss-Blanquet

  3. Microbiologie de l’Alimentation au Service de la Santé, MICALIS, UMR 1319, PAPPSO, INRA, 78352, Jouy-en-Josas, France

    Didier Chevret

  4. Architecture et Fonction des Macromolécules Biologiques, AFMB, UMR6098, CNRS, Aix-Marseille Université, 163 Avenue de Luminy, 13288, Marseille, France

    Pedro M. Coutinho & Bernard Henrissat

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  1. Laetitia Poidevin
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Correspondence to Eric Record.

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Poidevin, L., Berrin, JG., Bennati-Granier, C. et al. Comparative analyses of Podospora anserina secretomes reveal a large array of lignocellulose-active enzymes. Appl Microbiol Biotechnol 98, 7457–7469 (2014). https://doi.org/10.1007/s00253-014-5698-3

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  • DOI: https://doi.org/10.1007/s00253-014-5698-3

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