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Effect of angiotensin I-converting enzyme (ACE) inhibitory peptide purified from enzymatic hydrolysates of Styela plicata

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Abstract

Angiotensin I-converting enzyme (ACE) inhibitory peptide was isolated from Styela plicata. The S. plicata was hydrolyzed with various proteases including Protamex, Kojizyme, Neutrase, Flavourzyme, Alcalase, trypsin, α-chymotrypsin, pepsin, and papain. The hydrolysate prepared with Protamex had the highest ACE inhibitory activity compared to the other hydrolysates. We attempted to isolate ACE inhibitory peptides from hydrolysate prepared with Protamex using ultra-filtration, gel filtration on a Sephadex G-25 column and reversed-phase high-performance liquid chromatography (RP-HPLC) on an ODS column. IC50 value of the purified ACE inhibitory peptide was 24.7 μM, and Lineweaver–Burk plots suggest that the purified peptide from S. plicata acts as mixed-type inhibitor against ACE. Amino acid sequence of the purified peptide was identified as Met-Leu-Leu-Cys-Ser, with a molecular weight 566.4 Da. The results of this study suggest that peptides derived from S. plicata may be beneficial as anti-hypertension compounds in functional foods resource.

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Acknowledgments

This research was supported by a grant from the Korea Institute of Planning & Evaluation for Technology of Food, Agriculture, Forestry & Fisheries funded by Ministry of Food, Agriculture, Forestry & Fisheries, Korea.

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Authors and Affiliations

  1. Department of Marine Life Science, Jeju National University, Jeju, 690-756, Republic of Korea

    Seok-Chun Ko, Min Cheol Kang & You-Jin Jeon

  2. Faculty of Marine Bioscience and Technology, Kangnung-Wonju National University, Gangneung, 210-720, Republic of Korea

    Jung-Kwon Lee & Hee-Guk Byun

  3. Department of Chemistry, Pukyoung National University, Busan, 608-737, Republic of Korea

    Se-Kwon Kim

  4. Division of Food Science and Technology, Kyungnam University, Masan, 631-701, Republic of Korea

    Seung-Cheol Lee

  5. Korean Nokyong Research Center, College of Medicine, Konkuk University, Chungju, 380-701, Republic of Korea

    Byong-Tae Jeon

  6. Department of Biotechnology, College of Medicine, Konkuk University, Chungju, 380-701, Republic of Korea

    Pyo-Jam Park

  7. Department of Marine Life Science, Marine Life Research Center, Chosun University, Gwangju, 501-759, Republic of Korea

    Won-Kyo Jung

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  1. Seok-Chun Ko
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Correspondence to You-Jin Jeon.

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Ko, SC., Kang, M.C., Lee, JK. et al. Effect of angiotensin I-converting enzyme (ACE) inhibitory peptide purified from enzymatic hydrolysates of Styela plicata . Eur Food Res Technol 233, 915–922 (2011). https://doi.org/10.1007/s00217-011-1585-7

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