Ecological and Environmental Microbiology Original Articles

Annals of Microbiology

, Volume 59, Issue 3, pp 431-437

First online:

Antimicrobial potential of a marine seaweedAsparagopsis taxiformis againstLeptospira javanica isolates of rodent reservoirs

  • Kumaresan VedhagiriAffiliated withDivision of Medical Microbiology Department of Microbiology School of Life Sciences, Bharathidasan University
  • , Aseer ManilalAffiliated withDivision of Medical Microbiology Department of Microbiology School of Life Sciences, Bharathidasan University
  • , Thangavel ValliyammaiAffiliated withDivision of Medical Microbiology Department of Microbiology School of Life Sciences, Bharathidasan University
  • , Santhanam ShanmughapriyaAffiliated withDivision of Medical Microbiology Department of Microbiology School of Life Sciences, Bharathidasan University
  • , Sugathan SujithAffiliated withDivision of Medical Microbiology Department of Microbiology School of Life Sciences, Bharathidasan University
  • , Joseph SelvinAffiliated withDivision of Medical Microbiology Department of Microbiology School of Life Sciences, Bharathidasan University
  • , Kalimuthusamy NatarajaseenivasanAffiliated withDivision of Medical Microbiology Department of Microbiology School of Life Sciences, Bharathidasan University Email author 

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Abstract

In this present investigation pharmacologically active compounds were isolated from red algae (Asparagopsis taxiformis) and their efficacy was evaluated against theLeptospira javanica isolates of rodent carriers. The GC-MS analysis of the purified compound revealed the presence of 4,5-dimethyl-1H-pyrrole-2-carboxylic acid ethyl ester (56.012%), fattyacids, 14-methyl-pentadecanoic acid methyl ester (26.6%), octadecanoic acid methyl ester (8.46%), octadec-9-enoic acid 2,3-dihydroxy-propyl ester (4.11%), 9-octadecanoic acid, methyl ester (4.535%) and trace amount of chlorobenzene (0.09%). MICs and MBCs of the purified compound against pathogenic leptospiral strains belonging to 14 serovars and 11 isolates belonging to serovarjavanica ofLeptospira borgpetersenii were determined in a range of 100–1600 μg/ml. The antibiotics penicillin and doxycycline were used as the standards for the efficiency determination of the seaweed extract against the leptospiral reference serovars and isolates. The minimal inhibitory concentration of penicillin and doxycyline were in the range of 25–200 μg/ml. The seaweed active fraction exhibited comparable MIC and MBC values with that of the standard antibiotic doxycycline. In the present study the seaweed compound has been developed to apply for the 2nd nodal point of transmission cycle, the environment. Thus the present study draws the development of a novel drug to treat leptospires particularly in environments augmented with rodent carriers.

Key words

leptospirosis Asparagopsis taxiformis Leptospira borgpetersenii MIC MBC