Abstract
Goal, Scope and Background
In terrestrial ecotoxicology, standardised test methods using plants, earthworms and insects are available for the evaluation of effects induced by heavy metals, organic chemicals and, in particular, pesticides. Currently, these tests are performed either by using so-called Artificial Soils or (more or less) arbitrarily selected natural soils. Consequently, the test results depend not only on the intrinsic physico-chemical properties of the test chemical, but are also significantly influenced by the variable properties of the chosen soil In order to standardise the. test conditions and, at the same time, to relate the test results to representative soil types within the EU, it is proposed to modify the EURO-Soil concept for testing purposes.
Main Features
The EURO-Soil concept, i.e. the selection of a limited number of soils that are representative for Europe, was originally developed for the performance of standardised environmental fate tests. Despite many problems in detail, soils from six sites all over Europe were identified that cover a wide range of soil properties (e.g. texture, pH, organic matter content) and, therefore, very different conditions concerning the bioavailability and, in turn, the effects of chemicals. Obviously, the routine use of EURO-Soils as a control or test substrate would require large amounts of soil. Therefore, it is proposed to modify this concept in such a way that all soils similar to one of the six EUROSoils can be used for ecotoxicological tests.
Results and Discussion
It is assumed that the six EURO-Soils are representative for wide areas of the European Union, but at the same time it is neglected that some soils typical for, e.g. Northern Scandinavia, have to be identified in the future. All soils having similar properties (i.e. texture, pH, C/N ratio, and organic matter content) as one of the original EURO-Soils are called SIM-Soils. In this contribution, ‘ranges’ are proposed for four main properties and the six EUROSoils, thus allowing the identification of the SIM-Soils. However, since these properties cover a continuum, soils cannot be classified easily into a small number of classes; expert knowledge is required in order to decide whether a natural soil belongs to a certain SIM-Soil class or not. In the long run, this classification must take biological parameters like their suitability for standard test organisms into account as well.
Recommendation and Outlook
The soils selected so far (at least one for each EURO-Soil) are actually tested using different biological test methods. Further tests are necessary in order to decide which tests can be done in which soil and whether new test systems, e.g. covering acid soils, have to be developed. However, it is already clear that the standard test species differ distinctly regarding their sensitivity towards soil properties. It is recommended to use the SIMSoils in order to provide the authorities with more field-relevant, data when assessing chemicals in the terrestrial environment.
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References
Amorim MJ, Römbke J, Soares AMVM (2004a): Effect of the herbicide Phenmedipham and different soil properties in the enchytraeidsEnchytraeus albidusandEnchytraeus luxuriosus (in preparation1)
Amorim MJ, Ròmbke J, Soares AMVM (2004b): Effect of the herbicide Phenmedipham and different soil properties in the collembolansFolsomia candida andHypogastrura assimilis (in preparation2)
AG Boden (1996): Bodenkundliche Kartieranleitung, 4. Auflage. BGR, Hannover
Edwards PJ, Coulson JM (1992): Choice of Earthworm Species for Laboratory Tests. In: Ecotoxicology of Earthworms. Greig-Smith PW, Becker H, Edwards PJ, Heimbach F (eds). Intercept, Andover 36–43
Egeler P, Römbke J, Meller M, Knacker Th, Franke C, Studinger G, Nagel R (1997): Bioaccumulation of Lindane and Hexachlorobenzene by Tubificid sludgeworms (Oligochaeta) under standardised laboratory conditions. Chemosphere 35, 835–852
Fairbrother A, Glazebrock PW, van Straalen NM, Tarazona JV (2002): Test Methods to Determine Hazards of Sparingly Soluble Metal Compounds in Soils. SETAC Press, Pensacola, 97 pp
FOCUS (1996): Soil persistence models and EU registration. Final report of the work of the Soil Modelling Work Group of FOCUS (Forum for the Co-ordination of pesticide rate models and their Use). European Commission Document 7617/VI/96, 77 pp
Garcia MVB (2004): Effects of pesticides on soil fauna: Development of ecotoxicological test methods for tropical regions. Ecology and Development Series No. 19. Zentrum för Entwicklungsforschung, Universität Bonn, 281 pp
Gawlik BM, Sotiriou N, Kuhnt G, Karcher W, Kettrup A, Muntau H (1996): European reference soils as a common basis for soil testing of environmental chemicals in the EU. Fresenius Envir Bull 5, 610–618
Gawlik BM (1998): EURO-Soils II. Die neue Generation europäischer Referenzböden für Sorptionsstudien. S.P.I. 98.135. Environment Institute, Joint Research Centre, European Commission, 90 pp
Gawlik BM, Lamberty A, Muntau H, Pawels J (2001): EUROSoils — A set of CRMs for comparability of soil-measurements. Fresenius J Anal Chem 370, 220–223
Gawlik BM, Lamberty A, Pawels J, Blum WEH, Mentler A, Bussian B, Eklo O, Fox K, Kördel W, Hennecke D, Maurer T, PerrinGanier C, Pflugmacher J, Romero-Taboada E, Szabo G, Muntau H (2003): Certification of the European reference soil set (IRMM-443 — EURO-Soils). Part I. Adsorption coefficients for atrazine, 2,4-D and Lindane. The Science of the Total Environment 312, 23–31
ISO (International Organization for Standardization) (2002): Soil quality — Effects of pollutants on insect larvae (Oxythyrea funesta). Determination of acute toxicity using artificial soil substrate. ISO 20963, Geneve, Switzerland
Jessen-Hesse V, Terytze K, Römbke J, Jänsch S, Eisenträger A, Filzek P, Grabert E, Hüttner S, Hund-Rinke K, Jander JP, NeumannHensel H (2003): Preparation and performance of ecotoxicological test methods under practical conditions — A project overview and first results. Mittl Dtsch Bodenkundl Ges 102, 773–774
Jänsch S, Amorim MJ, Römbke J (2004): Identification of the ecological requirements of the most important terrestrial ecotoxicological test species (submitted3)
Kalsch W, Römbke J (1999): Zur chronischen Wirkung von TNT auf die StoppelrübeBrassica rapa im Labortest. In: Ökotoxikologie — Ökosystemare Ansätze und Methoden. Oehlamann J, Markert B (eds.) ecomed Verlag, Landsberg, 100-105 pp
Knacker T, Van Gestel CAM, Jones SE, Soares AMVM, Schallnass H-J, Förster B, Edwards CA (2004): Ring-testing and field-validation of a Terrestrial Model Ecosystem (TME) — An instrument for testing potentially harmful substances: Conceptual approach and study design. Ecotoxicology 13, 9–28
Kuhnt G, Gawlik BM, Muntau H (1999): The EURO-Soil-Project in the course of time: background and historical evolution. In: EURO-Soils II Laboratory Reference Materials for soil related studies. Gawlik BM, Muntau H (eds). Office for official publications of the European community, Luxembourg. 11–18
Kuhnt G, Muntau H (1992): European reference soils for sorption testing. Fresenius Environ Bull 1, 589–594
Løkke H, Gestel CAM (1998): Handbook of soil invertebrate toxicity tests. Wiley & Sons, Chichester, 281 pp
Løkke H, Janssen CR, Lanno RP, Römbke J, Rundgren S, Van Straalen NM (2002): Soil Toxicity Tests — Invertebrates. In: Test Methods to Determine Hazards of Sparingly Soluble Metal Compounds in Soils. Fairbrother A, Glazebrook PW, Van Straalen NM, Tarazona JV (eds). SETAC Press, Pensacola, USA, 128 pp
OECD (Organisation for Economic Co-Operation and Development) (1981): Guideline for Testing of Chemicals No. 106. Adsorption / Desorption. Paris
OECD (Organisation for Economic Co-Operation and Development) (1984): Guideline for Testing of Chemicals No. 207. Earthworm Acute Toxicity Test. Paris
OECD (Organisation for Economic Co-Operation and Development) (1995): Final Report of the OECD Workshop on Selection of Soils/Sediments. Belgirate, Italy 1995. Paris
OECD (Organisation for Economic Co-Operation and Development) (2003): Guideline for Testing of Chemicals. Proposal for Updating Guideline No. 208. Terrestrial Plant Test: Seedling, Emergence and Seedling Growth. Paris
Römbke J, Knacker T (2003): Standardisation of Terrestrial Ecotoxicological Effect Methods: An example of successful international co-operation. JSS -J Soils & Sediments 3, 237–238
Rundgren S, Nilsson P (1997): Sublethal effects of Aluminium on earthworms in acid soil: The usefulness ofDendrodrilus rubidus in a laboratory test system. Pedobiologia 41, 417–436
Schinkel K (1985): Prüfung der Beständigkeit von Pflanzenschutzmitteln im Boden im Rahmen des Zulassungsverfahrens. Ber Landwirt 198, 9–20
Stephenson GL, Feisthauer NC (2003): Eisenia Andrei reproduction in field-collected reference soils. Book of Abstracts. The 7th International Symposium on Earthworm Ecology, Cardiff, Wales 2002,p 73
Van Gestel CAM, Weeks JM (2004): Recommendations of the 3rd International Workshop on Earthworm Ecotoxicology, Aarhus, Denmark, August 2001. Ecotox Envir Safety 57, 100–105
Weissteiner C, Brandstetter A, Mentler A, Unterfrauner H, Wenzel WW, Blum WEH (1999): EURO-Soil 7 — A representative soil for alpine regions. In: EURO-Soils II — Laboratory reference Materials for soil-related studies. Gawlik BM, Muntau H (eds). Office for official publications of the European community, Luxembourg, 57–68
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Römbke, J., Amorim, M. Tackling the heterogeneity of soils in ecotoxicological testing an euro-soil based approach. J Soils & Sediments 4, 276–281 (2004). https://doi.org/10.1007/BF02991124
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DOI: https://doi.org/10.1007/BF02991124