Summary
Little is known about the nature of the mucosa-associated immune system within the normal colon, or about the immune response to colon carcinoma. In this study inflammatory cells (ICs) in 14 normal colons and 14 carcinomas were characterized. Overall inflammation, lymphocytes, plasma cells, neutrophils, and eosinophils were graded in routine H & E sections. Frozen sections were stained by an immunoperoxidase technique using antibodies to the T cell associated antigens CD2, CD7, CD4, CD8, and T cell receptors αβ and γδ. B cells were identified with CD20, macrophages with CD68, and Class II antigen with anti-HLA DR. Each cell type was semiquantitatively graded in 10 high power fields (HPFs) in the lumenal half (LH) or basal half (BH) of the normal mucosae, and in epithelium or stroma of the carcinomas. In normal colons, ICs were more frequent in LH than in BH. Plasma cells, lymphocytes and monocytes predominated. Subtyping of lymphocytes showed that CD4+ TCR αβ+ T lymphocytes were most numerous in the lamina propria. Lymphocytes within the epithelium were CD8+ T cells. Around carcinomas the overall grade of ICs was 1+ in the majority of cases. Plasma cells, CD4+ and CD8+ cells with the TCR αβ receptor, and macrophages were most frequent. Lymphoid aggregates of both T and B cells were frequent. Conclusions: 1. Normal colon contains a diffuse lumenally oriented population of TCR αβ+ CD4+ T cells, plasma cells, macrophages and class II antigen-expressing cells in the lamina propria. Intraepithelial lymphocytes are of the T suppressor phenotype. CD4+ T cells, macrophages and HLG-DR+ cells predominate in the response to colon carcinomas.
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References
Allen C, Hogg N (1985) Monocytes and other infiltrating cells in human colorectal tumours identified by monoclonal antibodies. Immunology 55:289–299
Allen C, Hogg N (1987) Elevation of infiltrating mononuclear phagocytes in human colorectal tumors. J Natl Cancer Inst 78:465–470
Balch CM, Riley LB, Bae YJ, Salmeron MA, Platsoucas CD, von Eschenbach A, Itoh K (1990) Patterns of human tumor-infiltrating lymphocytes in 120 human cancer. Arch Surg 125:200–205
Banner BF, Sonmez-Alpan E, Yousem SA (1993) An immunophenotypic study of the inflammatory cell populations in colon adenomas and carcinomas. Mod Pathol 6:295–301
Bland PW, Warren LG (1985) Immunohistologic analysis of the T-cell and macrophage infiltrate in 1,2-Dimethylhydrazine-induced colon tumors in the rat. J Natl Cancer Inst 75:757–764
Brandtzaeg P, Bosnes V, Halstensen TS, Scott H, Sollid LM Valnes KN (1998a) T lymphocytes in human gut epithelium preferentially express the A/B antigen receptor and are often CD45/UCHL1-Positive. Scand J Immunol 30:123–128
Brandtzaeg P, Halstensen TS, Kett K, Krajci P, Kvale D, Rognum TO, Scott H, Sollid LM (1989b) Immunobiology and immunopathology of human gut mucosa: humoral immunity and intraepithelial lymphocytes. Gastroenterology 97:1562–84
Csiba A, Whitwell HL, Moore M (1984) Distribution of histocompatibility and leukocyte differentiation antigens in normal human colon and in benign and malignant colonic neoplasms. Br J Cancer 50:699–709
Fenoglio-Preiser LM, Lantz PE, Listrom MB, Davis M, Rilke FO (1989) Gastrointestinal pathology. An atlas and text. Raven Press, New York, p 712
Halstensen TS, Scott H, Brandtzaeg P (1989) Intraepithelial T cells of the TCR G/DCD8+ and Vd1/Jd1+phenotypes are increased in coeliac disease. Scand J Immunol 30:665–672
Jennrich R, Sampson P (1988) General mixed model analysis of variance. In: Dixon WJ, Brown MB, Engelman L, Hill MA, Jennrich RI (eds) BMDP Statistical software manual, vol 2. U. Calif. Press, Berkeley, pp 1025–1045
Koch B, Giedl J, Hermanek P, Kalden JR (1985) The analysis of mononuclear cell infiltrations in colorectal adenocarcinoma. J Cancer Res Clin Oncol 109:142–151
Lennard TWJ, Warford A, Taylor RMR, Shenton BK, Proud G (1984) In situ subpopulations of lymphocytes in human colorectal carcinomas. Invasion Metastasis [Suppl] 4:60–66
McGhee JR, Mestecky J, Elson CO, Kiyono H (1989) Regulation of IgA synthesis and immune response by T cells and interleukins. J Clin Immunol 9:175–199
Miller CJ, McGhee JR, Gardner MB (1992) Mucosal immunity, HIV transmission, and AIDS. Lab Inves 68:129–145
Norazmi MN, Hohmann AW, Skinner JM, Jarvis LR, Bradley J (1990) Density and phenotype of tumour-associated mononuclear cells in colonic carcinomas determined by computer-assisted video image analysis. Immunology 69:282–286
Taunk J, Roberts AI, Ebert EC (1992) Spontaneous cytotoxicity of human intraepithelial lymphocytes against epithelial cell tumors. Gastroenterology 102:69–75
Umpleby HC, Heinemann D, Symes MO, Williamson RCN (1985) Expression of histocompatibility antigens and characterization of mononuclear cell infiltrates in normal and neoplastic colorectal tissues of humans. J Natl Cancer Inst 74:1161–1168
Vecchi M, Crosti L, Berti E, Agape D, Cerri A, DeFranchis R (1992) Increased jejunal intraepithelial lymphocytes bearing G/D T-cell receptor in dermatitis herpetiformis. Gastroenterology 102:1499–1505
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Banner, B.F., Savas, L., Baker, S. et al. Characterization of the inflammatory cell populations in normal colon and colonic carcinomas. Virchows Archiv B Cell Pathol 64, 213–220 (1993). https://doi.org/10.1007/BF02915115
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DOI: https://doi.org/10.1007/BF02915115