Summary
Mononuclear cell (MNC) populations in tissue specimens from 11 colorectal adenocarcinomas and 1 mucinous adenocarcinoma were analyzed, applying different monoclonal antibodies (moAB). Tumor epithelium could be characterized by MoAB VEP9, predominantly binding to mucus secreted by the tumor cells. In approximately 30%–50%, the tumor epithelium also reactdd in a patchy pattern with the MoABs OKT10 and OKIa, which define in the peripheral blood activated and HLA-DR expressing cells. T-lymphocytes, as defined by the MoAB 9.6, and T-lymphocyte subpopulations, as characterized by the MoABs OKT4 and OKT8, were found in the intratumoral stroma and in peritumoral inflammatory areas. Quantifying the relative amounts of mononuclear cell subpopulations in the different stroma compartments, a predominance of OKT10- and OKIa-defined and MoAB-S39-reactive cells was observed. NK cells, as labelled by the MoAB Leu-7, were only demonstrated singularly in different areas of the stroma. Nonlymphoid structures such as vessel walls were shown to express antigens recognized by the MoABs OKIa, OKT10, and Leu-7. In some instances, nerve structures were labelled by the MoABs OKIa, OKT10, S39, and Leu-7. Using semiquantitative analysis, no correlation could be obtained between the intratumoral and peritumoral infiltration with T-lymphocytes, T-lymphocyte subpopulations and monocytic cells, and histopathological tumor grading and staging.
Similar content being viewed by others
Abbreviations
- MoAB:
-
monoclonal antibody
- MNC:
-
mononuclear cell(s)
- PBS:
-
phosphate buffered saline
- PGE2:
-
prostaglandin E 2
References
Abo T, Balch CM (1981) A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J Immunol 127:1024–1029
Barclay AN, Mason DW (1982) Induction of Ia antigen in rat epidermal cells and gut epithelium by immunological stimuli. J Exp Med 156:1665–1676
Bröcker EB, Kolde G, Steinhausen D, Peters A, Macher E (1984) The pattern of the mononuclear infiltrate as a prognostic parameter in flat superficial spreading melanomas. J Cancer Res Clin Oncol 107:48–52
Ceuppens JL, Gualde N, Goodwin JS (1982) Phenotypic heterogeneity of the OKM1-positive lymphocyte population: reactivity of OKM1 monoclonal antibody with a subset of the suppressor/cytotoxic T-cell population. Cell Immunol 69:150–165
Daar AS, Fuggle SV, Ting A, Fabre JW (1982) Anomolous expression of HLA-DR antigens on human colorectal cancer cells. J Immunol 129:447–449
Dimitriu-Bona A, Burmester GR, Waters SJ, Winchester RJ (1983) Human mononuclear phagocyte differentiation antigens I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol 130:145–152
Evans R (1979) Host cells in transplanted murine tumors and their possible relevance to tumor growth. J Reticuloendothel Soc 26:427–437
Gooi HC, Thorpe SJ, Hounsell EF, Rumpold H, Kraft D, Förster O, Feizi T (1983) Marker of peripheral blood granulocytes and monocytes of man recognized by two monoclonal antibodies VEP8 and VEP9 involves the trisaccharide 3-fucosyl-N-acetyl-lactosamine. Eur J Immunol 13:306–312
Hefeneider SH, Conlon PJ, Henney CS, Gillis S (1983) In vivo interleukin 2 administration augments the generation of alloreactive cytolytic T lymphocytes and resisdent natural killer cells. J Immunol 130:222–227
Herberman RB, Holden HT (1979) Natural killer cells as antitumor effector cells. J Natl Cancer Inst 62:441–445
Hercend T, Ritz J, Schlossman SF, Reinherz EL (1981) Comparative expression of T9, T10 and Ia antigens on activated human T cell subsets. Human Immunol 3:247–259
Hermanek P, Gall FP, Altendorf A (1980) Prognostic groups in colorectal carcinoma. J Cancer Res Clin Oncol 98:185–193
Husby G, Strickland RG, Rigler GL, Peake GT, Williams RC (1977) Direct immunochemical detection of prostaglandin-E and cyclic nucleotides in human malignant tumors. Cancer 40:1629–1642
Introna M, Allavena P, Biondi A, Colombo N, Villa A, Mantovani A (1983) Defective natural killer activity within human ovarian tumors: low numbers of morphologically defined effectors present in situ. J Natl Cancer Inst 70:21–26
Kamoun M, Martin PJ, Hansen JA, Brown MA, Siadak AW, Nowinski RC (1981) Identification of a human T-lymphocyte surface protein associated with the E-rosette receptor. J Exp Med 153:207–212
Keller R (1980) Regulatory capacities of mononuclear phagocytes with particular reference to natural immunity against tumors. In: Herberman RB (ed) Natural cell-mediated immunity against tumors. Academic Press, New York, pp 1219–1269
Koch B, Locher P, Burmester GR, Mohr W, Kalden JR (1984a) The tissue architecture of synovial membranes in inflammatory and non-inflammatory joint diseases.II. The localization of mononuclear cells as detected by monoclonal antibodies directed against T-lymphocyte subsets and natural killer cells.Rheumatol Int 4:79–85
Koch B, Regnat W, Solbach W, Lanz R, Hermanek P, Kalden JR (1984b) Interleukin-2 production in peripheral blood mononuclear cells of patients with gastrointestinal tumors. J Clin Lab Immunol 13:171–178
Passwell JH, Dayler JM, Merler E (1979) Increased prostaglandin production by human monocytes after membrane receptor activation. J Immunol 123:115–120
Plescia OJ, Smith AH, Grinwich K (1975) Subversion of immune system by tumor cells and role of prostaglandins. Proc Nat Acad Sci 72:1849–1851
Pober JS, Gimbrone MA, Cotran RS, Reiss CS, Burakoff SJ, Fiers W, Ault KA (1983) Ia expression by vascular endothelium is inducible by activated T-cells and by human γ interferon. J Exp Med 157:1339–1353
Poppema, S, Bröcker EB, DeLeij L, Terbrack D, Visscher T, Terhaar A, Macher E, Thé TH, Sorg C (1983) In situ analysis of the mononuclear cell infiltrate in primary malignant melanoma of the skin. Clin Exp Immunol 51:77–82
Reinherz EL, Kung PC, Pesando JM, Ritz J, Goldstein G, Schlossman SF (1979) Ia determinants on human T-cell subsets defined by monoclonal antibody. J Exp Med 150:1472–1482
Reinherz EL, Schlossman SF (1980) The differentiation and function of human T-lymphocytes. Cell 19:821–827
Rumpold H, Kraft D, Obexer G, Radaszkiewicz T, Majdic O, Bettelheim P, Knapp W, Böck G (1983) Phenotypes of human large granular lymphocytes as defined by monoclonal antibodies. Immunobiol 164:51–62
Schuller-Petrovic S, Gebhart W, Lassmann H, Rumpold H, Kraft D (1983) A shared antigenic determinant between natural killer cells and nervous tissue. Nature 306:179–181
Selby WS, Janossy G, Bofill M, Jewell DP (1983a) Lymphocyte subpopulations in the human small intestine. The findings in normal mucosa and in the mucosa of patients with adult coeliac disease. Clin Exp Immunol 52:219–228
Selby WS, Janossy G, Mason DY, Jewell DP (1983b) Expression of HLA-DR antigens by colonic epithelium in inflammatory bowel disease. Clin Exp Immunol 53:614–618
Stein H, Bonk A, Tolksdorf G, Lennert K, Rodt H, Gerdes J (1980) Immunohistologic analysis of the organization of normal lymphoid tissue and non-Hodgkin's lymphomas. J Histochem Cytochem 28:746–760
Svennevig JL, Lövik M, Svaar H (1979) Isolation and characterization of lymphocytes and macrophages from solid, malignant human tumors. Int J Cancer 23:626–631
Tötterman TH, Parthenais E, Häyry P, Timonen T, Saksela E (1980) Cytological and functional analysis of inflammatory infiltrates in human malignant tumors. III. Further functional investigations using cultured autochthonous tumor cell lines and freezethawed infiltrating inflammatory cells. Cell Immunol 55:219–226
Walker C, Kristensen F, Bettens F, DeWeck AL (1983) Lymphokine regulation of activated (G1) lymphocytes. I. Prostaglandin E2-induced inhibition of interleukin 2 production. J Immunol 130:1770–1773
Wood GS, Warner NL, Warnke RA (1983) Anti-Leu-3/T4 antibodies react with cells of monocyte/macrophage and langerhans lineage. J Immunol 131:212–216
Wood GW, Goilahon KA (1977) Detection and Quantitation of macrophage infiltration into primary human tumors with the use of cell-surface markers. J Natl Cancer Inst 59:1081–1087
Author information
Authors and Affiliations
Additional information
Supported by DFG, SFB 118
Rights and permissions
About this article
Cite this article
Koch, B., Giedl, J., Hermanek, P. et al. The analysis of mononuclear cell infiltrations in colorectal adenocarcinoma. J Cancer Res Clin Oncol 109, 142–151 (1985). https://doi.org/10.1007/BF00391889
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00391889