Applied Microbiology and Biotechnology

, Volume 43, Issue 5, pp 786–793

Glycerol fermentation by a new 1,3-propanediol-producing microorganism:Enterobacter agglomerans

Authors

  • F. Barbirato
    • INRALaboratoire de Biotechnologie de l'Environment
  • C. Camarasa-Claret
    • INRA, Laboratoire de Microbiologie et de Biotechnologie des FermentationsInstitut des Produits de la Vigne
  • J. P. Grivet
    • CNRSCentre de Biophysique Moléculaire et Université d'Orléans
  • A. Bories
    • INRALaboratoire de Biotechnologie de l'Environment
Original Paper

DOI: 10.1007/BF02431909

Cite this article as:
Barbirato, F., Camarasa-Claret, C., Grivet, J.P. et al. Appl Microbiol Biotechnol (1995) 43: 786. doi:10.1007/BF02431909

Abstract

According to their ability to synthesize 1,3-propanediol from glycerol, two species were isolated from the anoxic mud of a distillery waste-water digestor:Clostridium butyricum andEnterobacter agglomerans. The latter, a facultatively anaerobic gram-negative bacterium, is described for the first time as a microorganism producing 1,3-propanediol from glycerol. The products of glycerol conversion byE. agglomerans were identified using nuclear magnetic resonance. A 20-g/l glycerol solution was fermented mainly to 1,3-propanediol (0.51 mol/mol) and acetate (0.18 mol/mol). Ethanol, formate, lactate and succinate were formed as by-products. Gas production was very low; 1,3-propanediol production perfectly balanced the oxido-reduction state of the microorganism. Acetate was the predominant metabolite generating energy for growth. High-glycerol-concentration fermentations (71 g/l and 100 g/l) resulted in an increase of the 1,3-propanediol yield (0.61 mol/mol) at the expense of lactate and ethamol production. Specific rates of glycerol consumption and 1,3-propanediol and acetate production increased whereas the growth rate decreased. The decreased in ATP yield was linearly correlated with the specific rate of 1,3-propanediol production. Incomplete glycerol consumption (about 40 g/l) was systematically observed when high glycerol concentrations were used. The unbalanced oxido-reduction state, the low carbon recovery and the detection of an unknown compound by HPLC observed in these cases indicate the formation of another metabolite, which is possibly an inhibitory factor.

Copyright information

© Springer-Verlag 1995