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Evaluation of instrumentation used for high performance thin-layer chromatography of aflatoxins

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Summary

Instrumentation used for high performance thin layer chromatography (HPTLC) of aflatoxins has been evaluated. Optimum sensitivity, accuracy and precision resulted when a combination of a fully automated TLC sampler, an unsaturated conventional TLC glass chamber and a monochromatic densitometer were employed. Benzene: acetonitrile (98: 2 v/v) was found to be the most suitable sample application solvent. These findings are being used to develop a rapid, simple and reproducible method for the analysis of aflatoxins.

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Part 1 of a series “Development of rapid methods for the analysis of aflatoxin in agricultural produce”.

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Coker, R.D., Jewers, K., Tomlins, K.I. et al. Evaluation of instrumentation used for high performance thin-layer chromatography of aflatoxins. Chromatographia 25, 875–880 (1988). https://doi.org/10.1007/BF02311421

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  • DOI: https://doi.org/10.1007/BF02311421

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