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A bi-directional HPTLC development method for the detection of low levels of aflatoxin in maize extracts

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Summary

A bi-directional high performance thin layer chromatography (HPTLC) method for the determination of low levels of aflatoxin in extracts of maize has been evaluated. The method, using diethylether and chloroform:xylene:acetone (6∶3∶1) as the two developing solvents was found to have a limit of detection of 0.8 (B1), 0.4 (B2), 1.7 (G1) and 0.4 (G2) μg/kg respectivety, with CVs equal or less than 1.7% (B1), 2.5% (B2), 3.1% (G1), and 3.1% (G2). Inter-plate variation was significantly greater than intra-plate variation.

Compared with two-dimensional HPTLC methods, a 15 to 30 fold improvement in the sample capacity per plate is achieved and positioning errors are much reduced during densitometry.

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Authors and Affiliations

  1. Overseas Development Natural Resources Institute, 56-62 Gray's Inn Road, WC1X 8LU, London, UK

    K. I. Tomlins, K. Jewers, R. D. Coker & M. J. Nagler

Authors
  1. K. I. Tomlins
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  2. K. Jewers
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  3. R. D. Coker
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  4. M. J. Nagler
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Tomlins, K.I., Jewers, K., Coker, R.D. et al. A bi-directional HPTLC development method for the detection of low levels of aflatoxin in maize extracts. Chromatographia 27, 49–52 (1989). https://doi.org/10.1007/BF02290404

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  • DOI: https://doi.org/10.1007/BF02290404

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