Abstract
The effect of STa enterotoxin on intracellular calcium in primary cultures of rat enterocytes was investigated. The enterocytes were loaded with a fluorescent calcium indicator, indo-1, and changes of intracellular calcium determined by flow cytometry. When the rat enterocytes were treated with EGTA, a calcium chelator, or ionomycin, a calcium ionophore, a decrease or increase, respectively, in free intracellular calcium was demonstrated. The addition of STa enterotoxin to rat enterocytes loaded with indo-1 caused an initial decrease, followed by a rapid increase, in free intracellular calcium. These effects on intracellular calcium occurred prior to or during an increase in the intracellular concentration of cyclic 3′,5′-guanosine monophosphate. The current results demonstrate that the activation of rat enterocytes by STa enterotoxin has an effect on transmembrane regulation of the receptor-dependent calcium signal and/or the release of calcium from intracellular storage sites.
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Knoop, F.C., Owens, M., Marcus, J.N. et al. Elevation of calcium in rat enterocytes byEscherichia coli heat-stable (STa) enterotoxin. Current Microbiology 23, 291–296 (1991). https://doi.org/10.1007/BF02092033
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DOI: https://doi.org/10.1007/BF02092033