Summary
The meiospore ofPhysoderma maydis (Phycomycetes, Chytridiales, Physodermataceae) has a nuclear cap enclosing the cellular ribosomes within a double membrane, and double membranes traversing the nuclear cap. Aggregates of ribosomes not incorporated into the nuclear cap are also enclosed by double membranes. A vesicular network is observed in the anterior portion of the spore in direct connection with the nuclear cap membrane and with a stacked parallel array of membranes, which itself is connected with the nuclear cap membrane.
The meiospore ofP. maydis contains a side body complex of the type observed in spores of theBlastocladiales. Vesicles enclose the side body complex and these vesicles are connected to the nuclear cap membrane and the nuclear envelope, and form a network which partially encloses the kinetosomal apparatus.
The nuclear cap membrane, stacked array of membranes, and the vesicles which surround the side body complex and the kinetosomal apparatus contain an electron-dense amorphous material. On the basis of their ultrastructural appearance, these membranes are interpreted as part of a highly divided microbody.
The ultrastructural organization of the meiospore ofP. maydis is compared to the structural organization observed in spores of theChytridiales, Blastocladiales, Monoblepharidales, andHarpochytriales. It is concluded that the structural organization of the meiospores ofP. maydis is the same as observed for members of theBlastocladiales, and it is suggested that thePhysodermataceae should be transferred from theChytridiales to theBlastocladiales.
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References
Anderson, P. J., 1967: Purification and quantitation of glutaraldehyde and its effect on several enzyme activities in skeletal muscle. J. Histochem. Cytochem.15, 652–661.
Barr, D. J. S., 1978: Taxonomy and phylogeny of chytrids. Biosystems10, 153–165.
—,Hartmann, V. E., 1976: Zoospore ultrastructure of threeChytridium species andRhizoclosmatium globosum. Canad. J. Bot.54, 2000–2013.
— —, 1978: The flagellar apparatus in theChytridiales. Canad. J. Bot.56, 887–900.
Chambers, J. C., Markus, K., Willoughby, L. C., 1967: The fine structure of mature zoosporangium ofNowakowskiella profusa. J. gen. Microbiol.46, 135–151.
Chong, J., Barr, D. J. S., 1974: Ultrastructure of the zoospores ofEntophlyctis confervaeglomeratae, Rhizophydium patellarium, andCatenaria anguillulae. Canad. J. Bot.52, 1197–1204.
Couch, J. N., 1953: The occurrence of thin-walled sporangia inPhysoderma zeae-maydis on corn in the field. J. Elisha Mitchell Sci. Soc.69, 182–184.
Dill, B. C., Fuller, M. S., 1971: Ammo acid immobilization of fungal motile cells. Arch. Mikrobiol.78, 92–98.
Eddins, A. H., 1933: Infection of corn plants byPhysoderma zeae-maydis. Shaw. J. Agric. Res.46, 241–253.
Fuller, M. S., 1966: Structure of the uniflagellate zoospores of aquatic Phycomycetes. Proc. Symp. Colston Res. Soc.18, 67–84.
—, 1977: The zoospore, hallmark of the aquatic fungi. Mycologia (U.S.)69, 1–20.
—,Calhoun, S. A., 1968: Microtubule kinetosome relationships in the motile cells of theBlastocladiales. Z. Zellforsch.87, 526–533.
—,Reichle, R., 1968: The fine structure ofMonoblepharella zoospores. Canad. J. Bot.46, 279–283.
—,Olson, L. W., 1971: The zoospore ofAllomyces. J. gen. Microbiol.66, 171–183.
Galey, F. R., Nilsson, S. E. G., 1966: A new method for transferring sections from the liquid surface of the trough through staining solutions to the supporting film of a grid. J. Ultrastruct. Res.14, 405–410.
Kazama, F. Y., 1972: Ultrastructure and phototaxis of the zoospores ofPhlyctochytrium sp., an estuarine chytrid. J. gen. Microbiol.71, 555–566.
Lange, L., Olson, L. W., 1976: The zoospore ofOlpidium brassicae. Protoplasma90, 33–47.
— —, 1977: The zoospore ofPhlyctochytrium aestuarii. Protoplasma93, 27–43.
Martin, W. W., 1971: The ultrastructure ofCoelomomyces punctatus zoospores. J. Elisha Mitchell Sci. Soc.87, 209–221.
McNitt, R., 1974: Ultrastructure ofPhlyctochytrium irregulare zoospores. Cytobiologie9, 307–320.
Mills, G. L., Cantino, E. C., 1975: The single microbody in the zoospore ofBlastocladiella emersonii is a “symphomicrobody”. Cell Differ.4, 35–43.
Miyabe, H., 1909: In: Handbook of plant diseases of Japan (Ideta, A., ed.), 4, Pt. 1, 1–334 (in Japanese).
Myers, R. B., Cantino, E. C., 1974: The gamma particle. In: Monographs in developmental biology (Wolsky, A., ed.), Vol. 8. Basel: S. Karger.
Olson, L. W., 1973: The meiospore ofAllomyces. Protoplasma78, 113–127.
—,Fuller, M. S., 1968: Ultrastructural evidence for the biflagellate origin of the uniflagellate fungal zoospore. Arch. Mikrobiol.62, 237–250.
—,Rønne, M., 1975: Induction of abnormal male gametes and androgenesis in the aquatic PhycomyceteAllomyces. Protoplasma84, 327–344.
—,Lange, L., Reichle, R., 1978: The zoospore and meiospore of the aquatic PhycomyceteCatenaria anguillulae. Protoplasma94, 53–71.
Powell, M. J., 1978: Phylogenetic implications of the microbody-lipid globule complex in zoosporic fungi. Biosystems10, 167–180.
Reichle, R., 1972: Fine structure ofOedogoniomyces zoospores with comparative observations onMonoblepharella zoospores. Canad. J. Bot.50, 819–824.
Reynolds, E. S., 1963: The use of lead citrate at highpH as an electron opaque stain in electron microscopy. J. Cell Biol.17, 208–212.
Schnepf, E., Deichgräber, G., Hegewald, E., Soeder, C. J., 1971: Elektronenmikroskopische Beobachtungen an Parasiten ausScenedesmus-Massenkulturen. 3.Chytridium sp. Arch. Mikrobiol.75, 230–245.
Sparrow, F. K., 1934: The occurrence of true sporangia in thePhysoderma disease of corn. Science (U.S.)79, 563–564.
—, 1947: Observations on chytridiaceous parasites of phanerograms. 11. A preliminary study of the occurrence of ephemeral sporangia in thePhysoderma disease of maize. Amer. J. Bot.34, 94–97.
—, 1974: Observations on chytridiaceous parasites of phanerograms. XXI. The endobiotic stage ofPhysoderma maydis. Mycologia (U.S.)66, 693–697.
Spurr, A. R., 1969: A low viscosity epoxy resin embedding media for electron microscopy. J. Ultrastruct. Res.26, 31–43.
Stuben, H., 1939: Über Entwicklungsgeschichte und Ernährungsphysiologie eines neuen niederen Phycomyceten mit Generationswechsel. Planta30, 353–383.
Tisdale, W. H., 1919:Physoderma disease of corn. J. Agric. Res.16, 137–154.
Teter, H. E., 1944: Isogamous sexuality in a new strain ofAllomyces. Mycologia (U.S.)36, 194–210.
Travland, L. B., 1977: Cytology ofCoelomomyces psorophorae: meiospore, gamete, zygote, and initiation of infection. Ph. D. thesis, Department of Botany, University of Washington, Seattle.
—,Whisler, H., 1971: Ultrastructure ofHarpochytrium hedinii. Mycologia (U.S.)63, 767–789.
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Olson, L.W., Lange, L. The meiospore ofPhysoderma maydis. The causal agent ofPhysoderma disease of maize. Protoplasma 97, 275–290 (1978). https://doi.org/10.1007/BF01276699
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DOI: https://doi.org/10.1007/BF01276699