Skip to main content
SpringerLink
Log in

Histochemistry of proteinases in the cercariae ofDiplostomum pseudospathaceum (Trematoda, Diplostomatidae)

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Two distinct endopeptidases were detected histochemically in the cercariae ofDiplostomum pseudospathaceum: a serine proteinase occupying the ceca and a cysteine proteinase occupying the penetration glands. The former was relatively resistant to formaldehyde, required either Ca2+ or Mg2+ for its stability, and was sensitive to organic fluorophosphate inhibitors but insensitive to thiol-blocking agents. The latter enzyme required both reducing and divalent cation-complexing agents for its full activity and was sensitive to formaldehyde, thiolblocking agents, and diazonium salts. Both enzymes hydrolyzed a number ofN-blockedl-aminoacyl-, andN-blockedl-peptidyl-naphthylamides bearingl-arginine at the P1 subsite. The pH optima for hydrolysis of the substrates were 8.0 for the serine proteinase and 7.6 for the cysteine proteinase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

TAPS :

N-tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid

MOPS :

3-(morpholino)-propanesulfonic acid

EDTA :

ethylenediaminetetraacetic acid

DTE :

dithioerythritol

ZN :

benzyloxycarbonylpHMB sodiump-hydroxymercuribenzoate

NEM :

N-ethylmaleimide

DFP :

diisopropyl fluorophosphate

PMSF :

phenylmethanesulfonyl fluoride

References

  • Dolbeare F, Vanderlaan M (1979) A fluorescent assay of proteinases in cultured mammalian cells. J Histochem Cytochem 27: 1493–1495

    PubMed  Google Scholar 

  • Lojda Z, Gossrau R, Schiebler TH (1979) Enzyme histochemistry. A laboratory manual. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Matsumura K, Shimada M, Sato K, Aoki Y (1990) Praziquantel-induced secretion of proteolytic enzyme fromSchistosoma mansoni cercariae. J Parasitol 76: 436–438

    PubMed  Google Scholar 

  • Moczoń T (1994) A cysteine proteinase in the cercariae ofDiplostomum pseudospathaceum (Trematoda, Diplostomatidae). Parasitol Res 80: 680–683

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Parasitology, Polish Academy of Sciences, Pasteura 3, P.O.B. 153, 00-973, Warsaw, Poland

    T. Moczoń

Authors
  1. T. Moczoń
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by research grant PB 6 6004 92 03 from the State Committee for Scientific Research.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moczoń, T. Histochemistry of proteinases in the cercariae ofDiplostomum pseudospathaceum (Trematoda, Diplostomatidae). Parasitol Res 80, 684–686 (1994). https://doi.org/10.1007/BF00932953

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00932953

Keywords

Search

Navigation