Abstract
Earlier labeling experiments have shown that autotrophically grown Acetobacterium woodii assimilates cell carbon via direct acetyl CoA formation from 2 CO2, rather than via the Calvin cycle. Cell extracts contained the enzymes required for biosynthesis starting from acetyl CoA and CO2. Notably, pyruvate synthase, pyruvate phosphate dikinase, and phosphoenolpyruvate carboxytransphosphorylase were present in sufficiently high activities. Ribulose-1,5-bisphosphate carboxylase activity could not be detected. The observed enzyme pattern was consistent with the postulated biosynthetic pathway as deduced from 14C-labeling experiments.
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Eden, G., Fuchs, G. Autotrophic CO2 fixation in Acetobacterium woodii . Arch. Microbiol. 135, 68–73 (1983). https://doi.org/10.1007/BF00419485
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DOI: https://doi.org/10.1007/BF00419485