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Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh

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Abstract

RAPD markers were used to assess genetic fidelity of 23 micropropagated plants of a single clone (L34) of Populus deltoides. Eleven arbitrary 10-base primers were successfully used to amplify DNA from in vivo and in vitro material. Of these, 5 distinguished a total of 13 polymorphisms common across 6 micropropagated plants. Apart from these 6 plants, the amplification products were monomorphic across all the micropropagated plants, the mother plant and 4 additional field-grown control plants. Our results show that RAPD markers can be used to gain rapid and precise information about genetic similarities or dissimilarities in micropropagation systems that might not be so easily evident from other commonly used techniques.

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Author notes

  1. Ajay Parida

    Present address: M.S. Swaminathan Research Foundation, Taramani Institutional Area, 600 113, Madras, India

Authors and Affiliations

  1. Laboratory of Cellular and Molecular Cytogenetics, Department of Botany, University of Delhi, 110 007, Delhi, India

    Vijay Rani, Ajay Parida & S. N. Raina

Authors
  1. Vijay Rani
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  2. Ajay Parida
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  3. S. N. Raina
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Communicated by G. C. Phillips

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Rani, V., Parida, A. & Raina, S.N. Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh. Plant Cell Reports 14, 459–462 (1995). https://doi.org/10.1007/BF00234055

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  • DOI: https://doi.org/10.1007/BF00234055

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