Abstract
A simple and efficient in vitro method for breaking the dormancy of Taxus baccata L. cv. Stricta seeds was investigated. The highest rate of germination (100%) of embryos isolated from seeds, which had been washed with running tap water for 7 days, was obtained after 7 days of culture on modified Murashige and Skoog or Heller media. The taxol equivalent content in 2-month-old Taxus plantlets was investigated using anti-taxol polyclonal antibodies. The results showed that the taxol equivalent content varied, depending on Taxus species and on the individuals in the same taxon.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Denis JN, Greene AE, Guenard D, Gueritt-Voegelin F, Mangatal L & Potier P (1988) A highly efficient practical approach to natural taxol. J. Amer. Chem. Soc. 110: 5917–5919
Flores HE & Sgrignoli PJ (1991) In vitro culture and precocious germination of Taxus embryos. In vitro Cell. Dev. Biol. 27: 139–142
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50: 148–151
Heller R (1953) Recherche sur la nutrition minérale des tissus végétaux cultivés in vitro. Ann. Sci. Nat. Bot. Biol. Veg. 14: 1–223
Jaziri M, Diallo BM, Vanhaelen MH, Vanhaelen-Fastre RJ, Zhiri A, Becu AG & Homès J (1991) Enzyme-linked immuno-sorbent assay for the detection and the semiquantitative determination of taxane diterpenoids related to taxol in Taxus sp. and tissue cultures. J. Pharm. Belg. 46: 93–99
Le Page-Degivry MT (1968) Mise en évidence d'une dormance associée à une immaturité de l'embryon chez Taxus baccata L. C.R. Acad. Sci. 266: 1028–1030
Le Page-Degivry MT (1969) Mise en évidence de l'acide (+) abscissique chez une Gymnosperme. C.R. Acad. Sci. 269: 2534–2536
Le Page-Degivry MT (1973) Etude en culture in vitro de la dormance embryonnaire chez Taxus baccata L. Biol. Plant 15: 264–269
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Rowinsky EK, Cazenave LA & Donehower FC (1990) Taxol: a novel investigational antimicrotubule agent. J. Natl. Cancer Inst. 82: 1247–1259
Wani MC, Taylor MHL, Wall ME, Coggon P & McPhail AT (1971) Plant antitumor agents, VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J. Amer. Chem. Soc. 93: 2325–3227
Wareing PF & Phillips IDJ (1981) Growth and Differentiation in Plants. 3rd ed.. Pergamon Press, Oxford New York Toronto Sydny Paris Frankfurt
Wheeler NC & Hehnen MT (1993) Taxol: a study in technology commercialization. J. Forestry 10: 15–18
White PR & Risser PG (1968) Nutritional requirements of spruce tumor cell in vitro. Biol. Plant. 17: 600–635
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zhiri, A., Jaziri, M., Homès, J. et al. Factors affecting the in vitro rapid germination of Taxus embryos and the evaluation of taxol content in the plantlets. Plant Cell Tiss Organ Cult 39, 261–263 (1994). https://doi.org/10.1007/BF00035979
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00035979