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The Janus Face of Resveratrol in Astroglial Cells

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Abstract

Astroglial cells are key modulators of neuropathology events. Resveratrol, a redox-active compound present in grapes and wine, has a wide range of biological effects. The aim of this study was to investigate whether resveratrol is able to prevent hydrogen peroxide (H2O2)-induced oxidative damage in C6 astroglial cells. We found that following a short oxidative insult (Model I—1 mM H2O2/30 min), resveratrol increased glutamate uptake (60%), glutamine synthetase (GS) (139%), glutathione (GSH) (120%), and S100B secretion (24%); and attenuated DCFH oxidation (34%) as compared to H2O2 values. Under less intense (0.1 mM H2O2), but lasting (6 h) insult (Model II), resveratrol had an opposite effect, potentiating the H2O2-induced decrease in glutamate uptake (from 34 to 63%), in GS (from 22 to 50%), in GSH (from 22 to 54%), and also potentiating DCFH oxidation (from 24 to 38%). The transcription factor, NF-κB, was activated in both models. Cell morphology alterations were also observed in the presence of H2O2 with process-bearing cells, accompanied by cell body retraction and actin reorganization. This effect was not prevented by resveratrol, but was prevented by lysophosphatidic acid (LPA), a specific upstream positive regulator of Rho A. In summary, these findings showed that resveratrol, a redox-active compound, was able to modulate important neurotrophic function of astroglial cells under different oxidative conditions.

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Acknowledgments

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and FINEP/Rede IBN 01.06.0842-00.

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  1. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600 anexo, Porto Alegre, RS, 90035-003, Brazil

    André Quincozes-Santos, Patrícia Nardin, Daniela Fraga de Souza, Daniel P. Gelain, José Cláudio Moreira, Carlos-Alberto Gonçalves & Carmem Gottfried

  2. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil

    Alexandra Latini

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  1. André Quincozes-Santos
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  2. Patrícia Nardin
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  3. Daniela Fraga de Souza
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  4. Daniel P. Gelain
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  7. Carlos-Alberto Gonçalves
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  8. Carmem Gottfried
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Correspondence to Carmem Gottfried.

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Quincozes-Santos, A., Nardin, P., de Souza, D.F. et al. The Janus Face of Resveratrol in Astroglial Cells. Neurotox Res 16, 30–41 (2009). https://doi.org/10.1007/s12640-009-9042-0

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