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FISH analysis for TET2 deletion in a cohort of 362 Brazilian myeloid malignancies: correlation with karyotype abnormalities

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Abstract

We investigated the prevalence of TET2 deletion by using a new FISH probe in a cohort of 362 Brazilian patients with myeloid neoplasms and their association with cytogenetic information (G-banding analysis). Normal karyotype was observed in 45.8 % of MDS (n = 44), 43.8 % of AML (n = 39) and 46.3 % of MPN (n = 82). Abnormalities of 4q24 (deletions, translocations or inversions) were associated with another chromosomal abnormality in four patients by G-banding analysis (2 MDS, 1 AML and 1 MPN). Interphase FISH analysis revealed deletion of TET2 in 21 patients (6 patients with abnormal karyotype and in 15 patients with normal karyotype). arrayCGH analysis revealed a cryptic deletion of the region 4q24 in all eight patients selected with myeloid malignancies (3 MDS, 1 AML and 4 MPN). Considering the significantly high cost of determining the mutational status of TET2 in patient samples by using conventional sequencing methods and sometimes the lack of regular use of SNP/aCGH array methodologies, FISH for the detection of TET2 abnormalities may become a potentially useful clinical tool. The search for alterations in TET2 gene may be important for the prediction of prognosis in normal/altered AML patients’ karyotype or in the disease evolution of patients with MNP and MDS.

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Acknowledgments

This investigation was supported by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) (2011/01647-2) and the National Institute of Science and Technology in Stem Cell and Cell Therapy, Ribeirão Preto, Brazil (Proc. 52462-7/2007-0).

Conflict of interest

All the authors reported no potential conflicts of interest.

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Authors and Affiliations

  1. Department of Internal Medicine, Division of Hematology, School of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brazil

    Fábio Morato de Oliveira, Antônio Roberto Lucena-Araujo, Ana Silvia Gouvêa de Lima, Roberto Passetto Falcão & Eduardo Magalhães Rego

  2. National Institute of Science and Technology in Stem Cell and Cell Therapy, Ribeirão Preto, Brazil

    Fábio Morato de Oliveira, Antônio Roberto Lucena-Araujo, Ana Silvia Gouvêa de Lima, Roberto Passetto Falcão & Eduardo Magalhães Rego

  3. Hematology Division, Hospital of Base of São José do Rio Preto, São Paulo, Brazil

    Carlos Eduardo Miguel

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  1. Fábio Morato de Oliveira
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Correspondence to Fábio Morato de Oliveira.

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de Oliveira, F.M., Miguel, C.E., Lucena-Araujo, A.R. et al. FISH analysis for TET2 deletion in a cohort of 362 Brazilian myeloid malignancies: correlation with karyotype abnormalities. Med Oncol 30, 483 (2013). https://doi.org/10.1007/s12032-013-0483-1

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