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Aldehyde and Xanthine Oxidase Activities in Tissues of Streptozotocin-Induced Diabetic Rats: Effects of Vitamin E and Selenium Supplementation

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Abstract

Effects of vitamin E and selenium supplementation on aldehyde oxidase (AO) and xanthine oxidase (XO) activities and antioxidant status in liver, kidney, and heart of streptozotocin (STZ)-induced diabetic rats were examined. AO and XO activities increased significantly after induction of diabetes in rats. Following oral vitamin E (300 mg/kg) and sodium selenite (0.5 mg/kg) intake once a day for 4 weeks, XO activity decreased significantly. AO activity decreased significantly in liver, but remained unchanged in kidney and heart of vitamin E- and selenium-treated rats compared to the diabetic rats. Total antioxidants status, paraoxonase-1 (PON1) and erythrocyte superoxide dismutase activities significantly decreased in the diabetic rats compared to the controls, while a higher fasting plasma glucose level was observed in the diabetic animals. The glutathione peroxidase activity remained statistically unchanged. Malondialdehyde and oxidized low-density lipoprotein levels were higher in the diabetic animals; however, these values were significantly reduced following vitamin E and selenium supplementation. In summary, both AO and XO activities increase in STZ-induced diabetic rats, and vitamin E and selenium supplementation can reduce these activities. The results also indicate that administration of vitamin E and selenium has hypolipidemic, hypoglycemic, and antioxidative effects. It decreases tissue damages in diabetic rats, too.

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Acknowledgements

This work was supported by a grant from Nutrition Research Center, Medical Faculty and Students’ Research Committee, Tabriz University of Medical Sciences. This is a report of a database from thesis entitled “Effect of Vitamin E and Selenium on Lipid Peroxidation in Diabetic Rats”.

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Authors and Affiliations

  1. Biochemistry Department, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Tayyebeh Ghaffari & Mohammad Nouri

  2. Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

    Tayyebeh Ghaffari

  3. Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran

    Amir Ata Saei & Mohammad-Reza Rashidi

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  1. Tayyebeh Ghaffari
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  2. Mohammad Nouri
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  3. Amir Ata Saei
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  4. Mohammad-Reza Rashidi
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Correspondence to Mohammad-Reza Rashidi.

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Ghaffari, T., Nouri, M., Saei, A.A. et al. Aldehyde and Xanthine Oxidase Activities in Tissues of Streptozotocin-Induced Diabetic Rats: Effects of Vitamin E and Selenium Supplementation. Biol Trace Elem Res 147, 217–225 (2012). https://doi.org/10.1007/s12011-011-9291-7

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  • DOI: https://doi.org/10.1007/s12011-011-9291-7

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