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Sox9 Is Upstream of MicroRNA-140 in Cartilage

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Abstract

MicroRNA-140 (miR-140) is specifically expressed in developing cartilage tissues. We have previously reported that miR-140 plays an important role during palatal cartilage development by modulating platelet-derived growth factor receptor alpha (pdgfra) in zebrafish. However, the regulatory mechanism of miR-140 in cartilage is still unknown. Using developing zebrafish, sox9a mutant (sox9a−/−) and sox9b mutant (sox9b−/−) zebrafish and SOX9 small interfering RNA in human chondrocytes, T/C-28 cells, we found that miR-140 is regulated by the cartilage master transcription regulator Sox9 in zebrafish and mammalian cells.

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References

  1. He, L., & Hannon, G. J. (2004). Nature Rev Genet., 5, 522–531.

    Article  CAS  Google Scholar 

  2. He, X., Eberhart, J. K., & Postlethwait, J. H. (2009). Journal of Cellular and Molecular Medicine, 13, 606–618.

    Article  CAS  Google Scholar 

  3. Berezikov, E., van de Guryev, V., Belt, J., Wienholds, E., Plasterk, R. H., & Cuppen, E. (2005). Cell, 12, 21–24.

    Article  Google Scholar 

  4. Ason, B., Darnell, D. K., Wittbrodt, B., Berezikov, E., Kloosterman, W. P., Wittbrodt, J., et al. (2006). PNAS, 103, 14385–14389.

    Article  CAS  Google Scholar 

  5. Tuddenham, L., Wheeler, G., Ntounia-Fousara, S., Waters, J., Hajihosseini, M. K., Clark, I., et al. (2006). FEBS Letters, 580, 4214–4217.

    Article  CAS  Google Scholar 

  6. Wienholds, E., Kloosterman, W. P., Miska, E., Alvarez-Saavedra, E., de Berezikov, E., Bruijn, E., et al. (2005). Science, 309, 310–311.

    Article  CAS  Google Scholar 

  7. Oh, C.-D., Maity, S. N., Lu, J. F., Zhang, J., Liang, S., Coustry, F., et al. (2010). PLoS ONE, 5, 1–12.

    Google Scholar 

  8. Akiyama, H., Chaboissier, M. C., Martin, J. F., Schedl, A., & de Crombrugghe, B. (2002). Genes & Development, 16, 2813–2828.

    Article  CAS  Google Scholar 

  9. Foster, J. W., Dominguez-Steglich, M. A., Guioli, S., Kwok, C., Weller, P. A., Stevanovic, M., et al. (1994). Nature, 372, 525–530.

    Article  CAS  Google Scholar 

  10. Wagner, T., Wirth, J., Meyer, J., Zabel, B., Held, M., Zimmer, J., et al. (1994). Cell, 79, 1111–1120.

    Article  CAS  Google Scholar 

  11. Yan, Y. L., Willoughby, J., Liu, D., Crump, J. G., Wilson, C., Miller, C. T., et al. (2005). Development, 132, 1069–1083.

    Article  CAS  Google Scholar 

  12. Hata, K., Nishimura, R., Muramatsu, S., Matsuda, A., Matsubara, T., Amano, K., et al. (2008). JCI., 118, 3098–3108.

    Article  CAS  Google Scholar 

  13. Furumatsu, T., Tsuda, M., Taniguchi, N., Tajima, Y., & Asahara, H. (2005). Journal of Biological Chemistry, 280, 8343–8350.

    Article  CAS  Google Scholar 

  14. Hattori, T., Coustry, F., Stephens, S., Eberspaecher, H., Takigawa, M., Yasuda, H., et al. (2008). Nuc Acids Res., 36, 3011–3024.

    Article  CAS  Google Scholar 

  15. Tardif, G., Hum, D., Pelletier, J. P., Duval, N., & Martel-Pelletier, J. (2009). BMC Musculoskeletal Disorders, 10, 148.

    Article  Google Scholar 

  16. Nakamura, Y., Yamamoto, K., He, X., Otsuki, B., Kim, Y., Murao, H., et al. (2011). Nature Communications 2:251. doi:10.1038/ncomms1242.

  17. Miyaki, S., Sato, T., Inoue, A., Otsuki, S., Ito, Y., Yokoyama, S., et al. (2010). Genes Dev, 24, 1173–1185.

  18. Li, L., Meng, T., Jia, Z., Zhu, G., & Shi, B. (2010). American Journal of Medical Genetics, 152A, 856–862.

    Article  CAS  Google Scholar 

  19. Kimmel, C. B., Ballard, W. W., Kimmel, S. R., Ullmann, B., & Schilling, T. F. (1995). Developmental Dynamics, 203, 253–310.

    Article  CAS  Google Scholar 

  20. Thisse, C., Thisse, B., Schilling, T. F., & Postlethwait, J. H. (1993). Development, 119, 1203–1215.

    CAS  Google Scholar 

  21. Nakamura, Y., Inloes, J. B., Katagiri, T., & Kobayashi T. (2011). Chondrocyte-specific microRNA-140 regulates endochondral bone development and targets Dnpep to modulate bone morphogenetic protein signaling. Molecular and Cellular Biology. 31, 3019–28.

    Article  CAS  Google Scholar 

  22. Kou, I., & Ikegawa, S. (2004). Journal of Biological Chemistry, 279, 50942–50948.

    Article  CAS  Google Scholar 

  23. Walker, M. B., & Kimmel, C. B. (2007). Biotechnic and Histochemistry, 82, 23–28.

    Article  CAS  Google Scholar 

  24. Wright, E., Hargrave, M. R., Christiansen, J., Cooper, L., Kun, J., Evans, T., et al. (1995). Nature Genetics, 9, 15–20.

    Article  CAS  Google Scholar 

  25. Kageyama, R., Niwa, Y., & Shimojo, H. (2009). Molecules and Cells, 27, 497–502.

    Article  CAS  Google Scholar 

  26. Monteys, A. M., Spengler, R. M., Wan, J., Tecedor, L., Lennox, K. A., Xing, Y., et al. (2010). RNA, 16, 495–505.

    Article  Google Scholar 

  27. Ying, S. Y., & Lin, S. L. (2006). Journal of Biological Sciences, 13, 5–15.

    CAS  Google Scholar 

  28. Lee, Y., Kim, M., Han, J., Yeom, K. H., Lee, S., Baek, S. H., et al. (2004). EMBO Journal, 23, 4051–4060.

    Article  CAS  Google Scholar 

  29. van de Wetering, M., & Clevers, H. (1992). EMBO Journal, 11, 3039–3044.

    Google Scholar 

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Acknowledgements

We thank Dr. Keiko Tamai at Case Western Reserve University, Cleveland, OH for her valuable discussions and to NIH grants HD22486 and RR020833 to JHP.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Shinshu University School of Medicine, Matsumoto, 390-8621, Japan

    Yukio Nakamura & Hiroyuki Kato

  2. Institute of Neuroscience, University of Oregon, Eugene, OR, 97403, USA

    Xinjun He & John H. Postlethwait

  3. Department of Orthopaedic Surgery, Osaka City University Graduate School of Medicine, Osaka, 545-8585, Japan

    Shigeyuki Wakitani

  4. Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA

    Tatsuya Kobayashi

  5. Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo, 108-8639, Japan

    Sumiko Watanabe & Atsumi Iida

  6. Department of Surgery and Bioengineering, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo, 108-8639, Japan

    Hideaki Tahara

  7. Howard Hughes Medical Institute, Departments of Orthopaedic Surgery and Genetics, Children’s Hospital Boston and Harvard Medical School, Boston, MA, 02115, USA

    Matthew L. Warman

  8. Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand

    Ramida Watanapokasin

Authors
  1. Yukio Nakamura
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  2. Xinjun He
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  3. Hiroyuki Kato
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  4. Shigeyuki Wakitani
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  5. Tatsuya Kobayashi
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  6. Sumiko Watanabe
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  8. Hideaki Tahara
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  10. Ramida Watanapokasin
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  11. John H. Postlethwait
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Corresponding author

Correspondence to Ramida Watanapokasin.

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Yukio Nakamura and Xinjun He have equal contributions.

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Nakamura, Y., He, X., Kato, H. et al. Sox9 Is Upstream of MicroRNA-140 in Cartilage. Appl Biochem Biotechnol 166, 64–71 (2012). https://doi.org/10.1007/s12010-011-9404-y

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  • DOI: https://doi.org/10.1007/s12010-011-9404-y

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