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Proteomic identification of differentially expressed proteins in mature and germinated maize pollen

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Abstract

The identification of proteins involved in pollen germination and tube growth is important for fundamental studies of fertility and reproduction in flowering plants. We used 2-DE and MALDI-TOF-MS to identify differentially expressed proteins in mature (P0) and 1-h germinated (P1) maize pollen. Among about 470 proteins separated in 2D gels, the abundances of 26 protein spots changed (up- or down-regulation) between P0 and P1. The 13 up-regulated protein spots were mainly involved in tube wall modification, actin cytoskeleton organization, energy metabolism, signaling, protein folding and degradation. In particular, pectin methylesterase, inorganic pyrophosphatase, glucose-1-phosphate uridylyltransferase and rab GDP dissociation inhibitor α are highly up-regulated, suggesting their potential role in pollen tube growth. The down-regulated 13 protein spots mainly include defense-related proteins, pollen allergens and some metabolic enzymes. This study would contribute to the understanding of the changes in protein expression associated with pollen tube development.

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Abbreviations

2D:

Two dimensional

2-DE:

Two-dimensional electrophoresis

ADK:

Adenosine kinase

MPP:

Mitochondrial-processing peptidase

MALDI-TOF:

Matrix-assisted laser desorption/ionization time-of-flight

Mr:

Molecular weight

MS:

Mass spectrometry

P0:

Mature pollen

P1:

Pollen germinated for 1 h

pI:

Isoelectric point

PMEs:

Pectin methylesterases

rab-GDI:

rab GDP dissociation inhibitor

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (project 30971705) by the Program for Science and Technology Innovation Talents in Universities of Henan Province (project 2008HASTIT005) and by Innovation Scientists and Technicians Troop Construction Projects of Henan Province (project 9410051003).

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Authors and Affiliations

  1. College of Life Science, Henan Agricultural University, 450002, Zhengzhou, China

    Yunhao Zhu, Pengfei Zhao, Xiaolin Wu & Wei Wang

  2. Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou, China

    Wei Wang

  3. Dipartimento di Scienze Ambientali ‘G. Sarfatti’, Università di Siena, Siena, Italy

    Monica Scali & Mauro Cresti

Authors
  1. Yunhao Zhu
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  2. Pengfei Zhao
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  3. Xiaolin Wu
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  4. Wei Wang
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  5. Monica Scali
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  6. Mauro Cresti
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Corresponding author

Correspondence to Wei Wang.

Additional information

Communicated by Z.-L. Zhang.

The authors Y. Zhu and P. Zhao contributed equally to this work.

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Zhu, Y., Zhao, P., Wu, X. et al. Proteomic identification of differentially expressed proteins in mature and germinated maize pollen. Acta Physiol Plant 33, 1467–1474 (2011). https://doi.org/10.1007/s11738-010-0683-7

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  • DOI: https://doi.org/10.1007/s11738-010-0683-7

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