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Sleeve Gastrectomy Induces Weight Loss in Diet-Induced Obese Rats Even if High-Fat Feeding Is Continued

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Abstract

Background

Sleeve gastrectomy (SG) has been used for the surgical treatment of morbid obesity as a first or definitive procedure with satisfactory results. The objective of this study in rats was to establish the effects of SG on weight loss depending on the post-surgical type of diet followed.

Methods

Thirty male Wistar rats were fed ad libitum during 3 months on a high-fat diet (HFD) to induce obesity. After this first phase, rats were subdivided in three groups of ten rats each and underwent a sham intervention, an SG, or no surgery but were pair-fed to the amount of food eaten by the animals of the SG group. At this time point, half of the animals in each group continued to be fed on the HFD, while the other half was switched to a normal chow diet (ND). Thus, the following subgroups were established: sham-ND, sleeve-ND, pair-fed-ND as well as sham-HFD, sleeve-HFD, and pair-fed-HFD. Body weight and food intake were recorded daily for 4 weeks. The feed efficiency rate (FER) was determined from weekly weight gains and caloric consumption during this period.

Results

Statistically significant (P < 0.05) differences in body weight were observed between the six experimental groups after 4 weeks of the interventions with rats in the sleeve-ND group experimenting the highest weight loss (−78.2 ± 10.3 g) and animals in the pair-fed-HFD group exhibiting the lowest weight reduction (−4.0 ± 0.1 g). Interestingly, the FER value of rats that underwent the SG and continued to be fed on a HFD was significantly (P < 0.05) lower than that of sham operated and pair-fed animals on the same diet.

Conclusion

The positive effects of SG on weight reduction are observed in obese rats submitted to the intervention and subsequently following an ND or even an HFD.

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Abbreviations

SG:

Sleeve gastrectomy

HFD:

High-fat diet

ND:

Normal chow diet

FER:

Feed efficiency rate

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Acknowledgements

This work was supported by Fondo de Investigaciones Sanitarias (FIS PS0902330) from the Spanish Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo. The authors gratefully acknowledge the valuable collaboration of all the staff of the breeding house of the University of Navarra, in particular Elena Ciordia and Juan Percaz, as well as the advice of Javier Guillén.

Conflict of interest statement

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Surgery, Clínica Universidad de Navarra, Pamplona, Spain

    Víctor Valentí & Javier A. Cienfuegos

  2. Department of Histology and Pathology, University of Navarra, Pamplona, Spain

    Marina Martín & María A. Burrell

  3. Metabolic Research Laboratory, University of Navarra, Pamplona, Spain

    Beatriz Ramírez, Javier Gómez-Ambrosi, Amaia Rodríguez, Victoria Catalán, Sara Becerril, Andoni Lancha & Gema Frühbeck

  4. CIBER Fisiopatología de la Obesidad y Nutrición, Instituto de Salud Carlos III, University of Navarra, Pamplona, Spain

    Beatriz Ramírez, Javier Gómez-Ambrosi, Amaia Rodríguez, Victoria Catalán, Sara Becerril, Javier A. Cienfuegos & Gema Frühbeck

  5. Department of Otorhinolaryngology, Clínica Universidad de Navarra, Pamplona, Spain

    Secundino Fernández

  6. Department of Endocrinology and Nutrition, Clínica Universidad de Navarra, University of Navarra, Avda. Pío XII, 36, 31008, Pamplona, Spain

    Gema Frühbeck

Authors
  1. Víctor Valentí
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  2. Marina Martín
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  3. Beatriz Ramírez
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  4. Javier Gómez-Ambrosi
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  5. Amaia Rodríguez
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  6. Victoria Catalán
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  7. Sara Becerril
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  9. Secundino Fernández
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  10. Javier A. Cienfuegos
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  11. María A. Burrell
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  12. Gema Frühbeck
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Corresponding author

Correspondence to Gema Frühbeck.

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Valentí, V., Martín, M., Ramírez, B. et al. Sleeve Gastrectomy Induces Weight Loss in Diet-Induced Obese Rats Even if High-Fat Feeding Is Continued. OBES SURG 21, 1438–1443 (2011). https://doi.org/10.1007/s11695-010-0277-x

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  • DOI: https://doi.org/10.1007/s11695-010-0277-x

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