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Influence of galactoglucomannan oligosaccharides on root culture of Karwinskia humboldtiana

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Abstract

Galactoglucomannan oligosaccharides (GGMOs) activity in K. humboldtiana root culture has been determined. GGMOs inhibited adventitious root growth and lateral root induction in contrast to IAA, IBA, and NAA stimulating effect in these processes. Similarly, the combination of GGMOs with natural auxins (IAA, IBA) evoked an inhibition of adventitious root growth and lateral root induction that depended on the oligosaccharides concentration and the type of auxin. The growth stimulating effect of the synthetic auxin, NAA, in adventitious roots was negatively affected by GGMOs, but they were without influence on lateral root induction. The presence of oligosaccharides triggered lateral root position on adventitious roots and the anatomy of adventitious roots (diameter, proportion of primary cortex to the central cylinder, number and size of primary cortical cells, intercellular spaces, and the number of starch grains in cells of primary cortex) in dependence on their coactions with auxin.

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Abbreviations

ANOVA:

Analysis of variance

d.p.:

Degree of polymerisation

2,4-D:

2,4-Dichlorophenoxyacetic acid

GA3 :

Gibberellic acid

GGMOs:

Galactoglucomannan oligosaccharides

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

LSD test:

Least significant difference test

NAA:

1-Naphthaleneacetic acid

SE:

Standard error

WPM:

Woody plant medium

References

  • Albersheim P, Darvill AG, McNeil M, Valent BS, Sharp JK, Nothnagel EA, Davis KR, Yamazaki N, Gollin DJ, York WS, Dudman WF, Darvill JE, Dell A (1983) Oligosaccharins: naturally occurring carbohydrates with biological regulatory functions. In: Ciferri O, Dure L (eds) Structure and functions of plant genomes III. Plenum, New York, pp 293–312

    Google Scholar 

  • Aldington S, Fry SC (1993) Oligosaccharins. Adv Bot Res 19:1–101

    Article  CAS  Google Scholar 

  • Altamura MM, Zaghi D, Salvi G, De Lorenzo G, Bellincampi D (1998) Oligogacturonides stimulate pericycle cell wall thickening and cell divisions leading to stoma formation in tobacco leaf explants. Planta 204:429–436

    Article  CAS  Google Scholar 

  • Auxtová O, Lišková D, Kákoniová D, Kubačková M, Karácsonyi Š, Bilisics L (1995) Effect of galactoglucomannan-derived oligosaccharides on elongation growth of pea and spruce stem segments stimulated by auxin. Planta 196:420–424

    Article  Google Scholar 

  • Auxtová-Šamajová O, Lišková D, Kákoniová D, Kubačková M, Karácsonyi Š, Bilisics L (1996) Inhibition of auxin stimulated short-term elongation growth of pea stem segments by galactoglucomannan-derived oligosaccharides. J Plant Physiol 147:611–613

    Google Scholar 

  • Bálványos I, Kursinszki L, Szőke É (2001) The effect of plant growth regulators on biomass formation and lobeline production of Lobelia inflata L. hairy root cultures. Plant Growth Regul 34:339–345

    Article  Google Scholar 

  • Bellincampi D, Salvi G, De Lorenzo G, Cervone F, Marfà V, Eberhard S, Darvill AG, Albersheim P (1993) Oligogacturonides inhibit the formation of roots on tobacco explants. Plant J 4:207–213

    Article  CAS  Google Scholar 

  • Beňová-Kákošová A, Digonnet C, Goubet F, Ranocha P, Jauneau A, Pesquet E, Barbier O, Zhang Z, Capek P, Dupree P, Lišková D, Goffner D (2006) Galactoglucomannans increase cell population density and alter the protoxylem/metaxylem tracheary element ratio in xylogenic cultures of Zinnia. Plant Physiol 142:696–709

    Article  PubMed  CAS  Google Scholar 

  • Biondi S, Lenzi C, Baraldi R, Bagni N (1997) Hormonal effects on growth and morphology of normal and hairy roots of Hyoscyamus muticus. J Plant Growth Regul 16:159–167

    Article  CAS  Google Scholar 

  • Bollmark M, Eliasson L (1986) Effects of exogenous cytokinins on root formation in pea cuttings. Physiol Plant 68:662–666

    Article  CAS  Google Scholar 

  • Capek P, Kubačková M, Alföldi J, Bilisics L, Lišková D, Kákoniová D (2000) Galactoglucomannan from the secondary cell wall of Picea abies L. Karst. Carbohydr Res 329:635–645

    Article  PubMed  CAS  Google Scholar 

  • Côté F, Hahn MG (1994) Oligosaccharins: structures and signal transduction. Plant Mol Biol 26:1379–1411

    Article  PubMed  Google Scholar 

  • Denarie J, Debelle F, Prome JC (1996) Rhizobium lipochitooligosaccharide nodulation factors: signaling molecules mediating recognition and morphogenesis. Annu Rev Biochem 65:503–535

    Article  PubMed  CAS  Google Scholar 

  • Dey PM (1980) d-galactoglucomannans. Adv Carbohydr Chem Biochem 37:337–339

    Article  Google Scholar 

  • Eberhard S, Doubrava N, Marfà V, Mohnen D, Southwick A, Darvill AG, Albersheim P (1989) Pectic cell wall fragments regulate tobacco thin-cell-layer explants morphogenesis. Plant Cell 1:747–755

    Article  PubMed  CAS  Google Scholar 

  • Etzler ME (1998) Oligosaccharide signaling of plant cells. J Cell Biochem Suppl 30–31:123–128

    Article  PubMed  Google Scholar 

  • Guadagnini M, Herzig R, Erismann KH, Műller-Schärer H (1999) In vitro-Zűchtung, Selection und Erprobung von metall-akkumulierenden Tabakvarianten zur Bodensanierung. Terra Tech 6:52–54

    Google Scholar 

  • Gyulai G, Kiss E, Kiss J, Heszky LE (1993) Hormone-selective bioassay for auxins and cytokinins in vitro. Hung Agri Res 2:13–17

    Google Scholar 

  • Henselová M, Lux A, Masarovičová E (2002) Effect of growth regulators on rooting cuttings of Karwinskia species under in vivo conditions. Rostlinná výroba 48:471–476

    Google Scholar 

  • Husen A, Pal M (2001) Clonal propagation of Tectona grandis (Linn. f.): effects of IBA and leaf area on carbohydrates drifts and adventitious root regeneration on branch cuttings. Ann For 9:88–95

    Google Scholar 

  • Inada S, Shimmen T (2000) Regulation of elongation growth by gibberellin in root Lemna minor. Plant Cell Physiol 41:932–939

    Article  PubMed  CAS  Google Scholar 

  • Ishihara M, Nagao Y, Shimizu K (1991) Physiological activity of xylooligosaccharides. Res Rep Biomass Conv Program 27:20–32

    CAS  Google Scholar 

  • Ishii K, Kinoshita I, Shigenaga H, Ishihara M, Shimizu K, Nishihara H, Tanaka K (1992) The effects of oligosaccharides on tissue culture of white birch and black pine. Trans Japan For Soc 103:485–486

    Google Scholar 

  • Iwasaki I, Matsubara Y (2000) Purification of pectate oligosaccharides showing root-growth-promoting activity in lettuce using ultrafiltration and nanofiltration membranes. Biosci Bioeng 89:495–497

    Article  CAS  Google Scholar 

  • John M, Röhrig H, Schmidt J, Walden R, Schell J (1997) Cell signalling by oligosaccharides. Trends Plant Sci 2:111–115

    Article  Google Scholar 

  • Kaku T, Tabuchi A, Wakabayashi K, Hoson T (2004) Xyloglucan oligosaccharides cause cell wall loosening by enhancing xyloglucan endotransglucosylase/hydrolase activity in azuki bean epicotyls. Plant Cell Physiol 45:77–82

    Article  PubMed  CAS  Google Scholar 

  • Kim YS, Hahn EJ, Yeung EC, Paek KY (2003) Lateral root development and saponin accumulation as affected by IBA or NAA in adventitious root cultures of Panax ginseng CA Meyer. In Vitro Cell Dev Biol Plant 39:245–249

    Article  CAS  Google Scholar 

  • Kollárová-Sadloňová K, Lux A, Lišková D, Henselová M, Kákoniová D, Masarovičová E (2003) Rooting, root growth and root morphology of two Karwinskia species in vivo and in vitro. Biologia 58:89–94

    Google Scholar 

  • Kollárová K, Lišková D, Kákoniová D, Lux A (2004) Effect of auxins on Karwinskia humboldtiana root culture. Plant Cell Tissue Organ Cult 79:213–221

    Article  Google Scholar 

  • Kollárová K, Lišková D, Henselová M (2005) Effect of auxins and plant oligosaccharides on the root formation and elongation growth of mung bean hypocotyls. Plant Growth Reg 46:1–9

    Article  CAS  Google Scholar 

  • Kollárová K, Lišková D, Capek P (2006) Further biological characteristics of galactoglucomannan oligosaccharides. Biol Plant 50:232–238

    Article  Google Scholar 

  • Kubota H, Sato K, Yamada T, Maitani T (2000) Phytochelatin homologs induced in hairy roots of horseradish. Phytochemistry 53:239–245

    Article  PubMed  CAS  Google Scholar 

  • Lišková D, Ordóñez JR, Lux A, Piñeyro-López A (1994) Tissue culture of Karwinskia humboldtiana—a plant producing toxins with antitumoural effects. Plant Cell Tissue Organ Cult 36:339–343

    Article  Google Scholar 

  • Lišková D, Auxtová O, Kákoniová D, Kubačková M, Karácsonyi Š, Bilisics L (1995) Biological activity of galactoglucomannan-derived oligosaccharides. Planta 196:425–429

    Article  Google Scholar 

  • Lišková D, Lux A, Piñeyro-López A, Luján-Rangel R (1999) Karwinskia species (buckthorn): in vitro culture, micropropagation, and the production of toxin anthracenone. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, medicinal and aromatic plants, vol XI. Springer-Verlag, Berlin, pp 223–242

    Google Scholar 

  • Lozovaya VV, Zabotina OA, Rumyantseva NI, Malihov RG, Zihareva MV (1993) Stimulation of root development on buckwheat thin cell-layer explants by pectic fragments from pea stem cell walls. Plant Cell Rep 12:530–533

    Article  Google Scholar 

  • Lundqvist J, Teleman A, Junel L, Yacchi G, Dahlman O, Tjerneld F, Stalbrand H (2002) Isolation and characterization of galactoglucomannan from spruce (Picea abies). Carbohydr Polym 48:29–39

    Article  CAS  Google Scholar 

  • Lux A (1981) A rapid method for staining semithin sections of plant material. Biologia 36:753–757 (in Slovak)

    Google Scholar 

  • Poupart J, Waddell CS (2000) The rib1 is resistant to indole-3-butyric acid, an endogenous auxin in Arabidopsis. Plant Physiol 124:1739–1751

    Article  PubMed  CAS  Google Scholar 

  • Schröder R, Wegrzyn TF, Bolitho KM, Redgwell RJ (2004) Mannan transglycosylase: a novel enzyme activity in cell walls of higher plants. Planta 219:590–600

    Article  PubMed  CAS  Google Scholar 

  • Spiro MD, Bowers JF, Cosgrove DJ (2002) A comparison of oligogalacturonide- and auxin-induced extracellular alkalinization and growth responses in roots of intact cucumber seedlings. Plant Physiol 130:895–903

    Article  PubMed  CAS  Google Scholar 

  • Suzuki T, Tomita-Yokotani K, Tsubura H, Yoshida S, Kusakabe I, Yamada K, Yoichi M, Hasegawa K (2002) Plant growth-promoting oligosaccharides produced from tomato waste. Bioresour Technol 81:91–96

    Article  PubMed  CAS  Google Scholar 

  • Vuylsteker C, Dewaele E, Rambour S (1998) Auxin induced lateral root formation in chicory. Ann Bot 81:449–454

    Article  CAS  Google Scholar 

  • Warneck H, Seitz HU (1993) Inhibition of gibberellic acid-induced elongation-growth of pea epicotyls by xyloglucan oligosaccharides. J Exp Bot 44:1105–1109

    Article  CAS  Google Scholar 

  • Zabotina OA, Gurjanov OP, Ayupova DA, Lozovaya VV, Beldman G, Voragen AGJ (1997) Isolation and analysis of soluble oligosaccharides stimulating root formation from pea shoots. Biochemistry (Mosc) 62:845–850

    CAS  Google Scholar 

  • Zabotina OA, Ibragimova NN, Zabotin AI, Trofimova OI, Sitnikov AP (2002) Biologically active oligosaccharides from pectins of Pisum sativum L. seedlings affecting root generation. Biochemistry (Mosc) 67:227–232

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to Dr. M. Čiamporová, PhD., Department of Plant Physiology, Institute of Botany, Slovak Academy of Sciences for enabling the use of Leica Q500MC image analysis system QWin, and M. Nadubinská, PhD. for assistance with the image analysis system. This study was supported by the Slovak Grant Agency for Science (No. 2/7048/27) and Agency for the Support of Research and Development (No. APVT-51-013304).

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Authors and Affiliations

  1. Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, 845 38, Slovakia

    Karin Kollárová, Desana Lišková & Alexander Lux

  2. Department of Plant Physiology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina B-2, Bratislava, 842 15, Slovakia

    Alexander Lux

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  1. Karin Kollárová
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  2. Desana Lišková
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  3. Alexander Lux
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Correspondence to Desana Lišková.

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Kollárová, K., Lišková, D. & Lux, A. Influence of galactoglucomannan oligosaccharides on root culture of Karwinskia humboldtiana . Plant Cell Tiss Organ Cult 91, 9–19 (2007). https://doi.org/10.1007/s11240-007-9272-x

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  • DOI: https://doi.org/10.1007/s11240-007-9272-x

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