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Carbon acquisition by diatoms

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Abstract

Diatoms are responsible for up to 40% of primary productivity in the ocean, and complete genome sequences are available for two species. However, there are very significant gaps in our understanding of how diatoms take up and assimilate inorganic C. Diatom plastids originate from secondary endosymbiosis with a red alga and their Form ID Rubisco (ribulose-1,5-bisphosphate carboxylase-oxygenase) from horizontal gene transfer, which means that embryophyte paradigms can only give general guidance as to their C acquisition mechanisms. Although diatom Rubiscos have relatively high CO2 affinity and CO2/O2 selectivity, the low diffusion coefficient for CO2 in water has the potential to restrict the rate of photosynthesis. Diatoms growing in their natural aquatic habitats operate inorganic C concentrating mechanisms (CCMs), which provide a steady-state CO2 concentration around Rubisco higher than that in the medium. How these CCMs work is still a matter of debate. However, it is known that both CO2 and HCO −3 are taken up, and an obvious but as yet unproven possibility is that active transport of these species across the plasmalemma and/or the four-membrane plastid envelope is the basis of the CCM. In one marine diatom there is evidence of C4-like biochemistry which could act as, or be part of, a CCM. Alternative mechanisms which have not been eliminated include the production of CO2 from HCO −3 at low pH maintained by a H+ pump, in a compartment close to that containing Rubisco.

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Acknowledgements

Unpublished work reported here was performed under grant NER/A/S/2001/01130 from the Natural Environment Research Council, UK. We are grateful to Professor D G Mann for pointing out the work of Schmid (2001), and to the comments of two anonymous reviewers which have significantly improved the paper.

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  1. Plant Research Unit, University of Dundee at SCRI, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK

    Karen Roberts & John A. Raven

  2. Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK

    Espen Granum & Richard C. Leegood

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  1. Karen Roberts
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Roberts, K., Granum, E., Leegood, R.C. et al. Carbon acquisition by diatoms. Photosynth Res 93, 79–88 (2007). https://doi.org/10.1007/s11120-007-9172-2

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