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Quinoprotein Adducts Accumulate in the Substantia Nigra of Aged Rats and Correlate with Dopamine-Induced Toxicity in SH-SY5Y Cells

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Abstract

Parkinson’s disease (PD) is an age-dependent neurodegenerative disorder characterized by dopaminergic neuron loss in substantia nigra. Previous studies have implicated a role of dopamine oxidation in PD. Dopamine oxidation leads to the formation of dopamine quinone, which generates reactive oxygen species and covalently modifies cysteinyl proteins to form quinoprotein adduct. We compared quinoprotein adduct formation and lipid peroxidation in different brain regions of young and old rats. We found a prominent age-dependent accumulation of quinoprotein adducts in the substantia nigra, while no significant change of lipid peroxidation was detected in any brain regions of 2- to 15-month old rats. To determine whether quinoprotein adduct formation correlates with dopamine-induced cytotoxicity, we analyzed dopamine treated SH-SY5Y cells and found a strong correlation between quinoprotein adduct formation and cytotoxicity. Together, our results indicate that quinoprotein adduct formation may play a role in the age-dependent selective vulnerability of dopaminergic neurons in PD.

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Acknowledgments

This work was supported by the Ministry of Education of China project 985.

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Authors and Affiliations

  1. School of Life Science, East China Normal University, Zhongshan bei lu 3663, Shanghai, 200062, China

    Na Wang, Yan Wang, Guohua Yu, Chonggang Yuan & Jiyan Ma

  2. Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, OH, USA

    Jiyan Ma

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  1. Na Wang
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  2. Yan Wang
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  3. Guohua Yu
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  4. Chonggang Yuan
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  5. Jiyan Ma
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Correspondence to Chonggang Yuan or Jiyan Ma.

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Wang, N., Wang, Y., Yu, G. et al. Quinoprotein Adducts Accumulate in the Substantia Nigra of Aged Rats and Correlate with Dopamine-Induced Toxicity in SH-SY5Y Cells. Neurochem Res 36, 2169–2175 (2011). https://doi.org/10.1007/s11064-011-0541-z

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