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Differential effects of platelet rich plasma and washed platelets on the proliferation of mouse MSC cells

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Abstract

Multipotent mesenchymal stem cell (MSC) therapies are being tested clinically for a variety of disorders. However, despite the remarkable clinical advancements in this field, most applications still use traditional culture media containing fetal bovine serum. Platelet-rich plasma (PRP) appears as a novel application for tissue engineering and its effect on bone healing is thought to be mainly dependent on the proliferation promoting function, with the molecular mechanisms largely unknown. In this study, mouse osteogenic progenitor mesenchymal stem cells (MSCs) were cultured in PRP or washed platelet (WPLT)-treated wells or in untreated wells, and analyzed on cycloxygenase 2 (COX2) expression (qRT-PCR), cell growth (MTT assay) and cell differentiation (alkaline phosphatase activity). The results showed that PRP and WPLT stimulated cell growth similarly in the first 6 days, together with the steady induction of COX2 and PGE2. 10 μmol/l celecoxib (an inhibitor of COX2) significantly inhibited the pro-proliferation effects. Interestingly, WPLT had stronger effects than PRP in proliferation at the later time points (6–9 days). ALP activity assay and collagen 1a expression revealed PRP had a mild but statistically significant pro-differentiation effect, while no obvious effects observed in WLPT group. In summary, PRP stimulates initial growth of MSCs in a COX2 partially dependent manner and the less obvious osteogenic differentiation promoting effects of WPLT strongly indicates WPLT rather than the PRP should be the optional choice for expanding MSCs in vitro for clinical use.

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Acknowledgments

We thank Prof. Yan Jin(Research and Development Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi’an, 710032 Shaanxi, People’s Republic of China)This study was funded by National Science Foundation of China, NSFC30900860 to Dr Wei Kuang.

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Authors and Affiliations

  1. Department of Stomatology, Guangzhou General Hospital of Guangzhou Military Command, 111 Liuhua Road, 510010, Guangzhou, People’s Republic of China

    Jianmin Duan, Wei Kuang, Hongtao Li & Yi Zhang

  2. Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, No.145, Changlexi Road, 710032, Xi’an, Shaanxi, People’s Republic of China

    Jiali Tan

  3. Division of Endodontics, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Sakado, Japan

    Kikuchi Hirotaka & Katayama Tadashi

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  1. Jianmin Duan
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  2. Wei Kuang
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  3. Jiali Tan
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  4. Hongtao Li
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  5. Yi Zhang
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  6. Kikuchi Hirotaka
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  7. Katayama Tadashi
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Correspondence to Wei Kuang.

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Duan, J., Kuang, W., Tan, J. et al. Differential effects of platelet rich plasma and washed platelets on the proliferation of mouse MSC cells. Mol Biol Rep 38, 2485–2490 (2011). https://doi.org/10.1007/s11033-010-0385-7

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  • DOI: https://doi.org/10.1007/s11033-010-0385-7

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