Abstract
A polar bacterium was isolated from Arctic sea sediments and identified as Psychromonas artica, based on 16S rDNA sequence. Psychromonas artica KOPRI 22215 has an optimal growth temperature of 10 °C and a maximum growth temperature of 25 °C, suggesting this bacterium is a psychrophile. Cold shock proteins (Csps) are induced upon temperature downshift by more than 10 °C. Functional studies have researched mostly Csps of a mesophilic bacterium Escherichia coli, but not on those of psychrophilic bacteria. In an effort to understand the molecular mechanisms of psychrophilic bacteria that allow it withstand freezing environments, we cloned a gene encoding a cold shock protein from P. artica KOPRI 22215 (CspAPa) using the conserved sequences in csp genes. The 204 bp-long ORF encoded a protein of 68 amino acids, sharing 56% homology to previously reported E. coli CspA protein. When CspAPa was overexpressed in E. coli, it caused cell growth-retardation and morphological elongation. Interestingly, overexpression of CspAPa drastically increased the host’s cold-resistance by more than ten times, suggesting the protein aids survival in polar environments.
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Abbreviations
- Csp:
-
Cold shock protein
- CspAPa :
-
Cold-shock protein A from Psychromonas artica KOPRI 22215
- ssDNA:
-
Single-stranded DNA
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Acknowledgements
The authors greatly appreciate the gift of E. coli BX04 from Dr. Sangita Phadtare and Dr. Massayori Inouye (UMDNJ). This work was supported by the faculty research fund of Sejong University in 2008.
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Jung, Y.H., Yi, JY., Jung, H.J. et al. Overexpression of Cold Shock Protein A of Psychromonas arctica KOPRI 22215 Confers Cold-Resistance. Protein J 29, 136–142 (2010). https://doi.org/10.1007/s10930-010-9233-9
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DOI: https://doi.org/10.1007/s10930-010-9233-9