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Association Study of Common Genetic Variants in Pre-microRNAs in Patients with Ulcerative Colitis

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Abstract

Background

Common single-nucleotide polymorphisms (SNPs) in microRNAs (miRNA) have been shown to be associated with susceptibility to several human diseases. We evaluated the associations of three SNPs (rs11614913, rs2910164, and rs3746444) in pre-miRNAs (miR-196a2, miR-146a, and miR-499) with the risk of ulcerative colitis (UC) in a Japanese population.

Methods

The rs11614913 (T > C), rs2910164 (C > G), and rs3746444 (A > G) SNPs were genotyped in 170 UC and 403 control subjects.

Results

The rs3746444 AG genotype was significantly higher among the UC group (odds ratio (OR) = 1.51, 95% CI = 1.03–2.21, p = 0.037). The rs3746444 AG genotype was associated with onset at an older age (OR = 1.70, 95% CI = 1.04–2.78, p = 0.035), left-sided colitis and pancolitis (left-sided colitis, OR = 2.10, 95% CI = 1.12–3.94, p = 0.024; pancolitis, OR = 1.81, 95% CI = 1.09–3.01, p = 0.028, left-sided colitis + pancolitis, OR = 1.91, 95% CI = 1.26–2.92, p = 0.003), higher number of times hospitalized (OR = 2.63, 95% CI = 1.22–5.69, p = 0.017), steroid dependence (OR = 2.63, 95% CI = 1.27–5.44, p = 0.014), and refractory phenotypes (OR = 2.76, 95% CI = 1.46–5.21, p = 0.002) while the rs3746444 AA genotype was inversely associated with the number of times hospitalized (2∼, OR = 0.36, 95% CI = 0.17–0.79, p = 0.012), steroid dependence (OR = 0.42, 95% CI = 0.21–0.88, p = 0.021), and refractory phenotypes (OR = 0.38, 95% CI = 0.20–0.72, p = 0.003). The rs1161913 TT genotype also held a significantly higher risk of refractory phenotype (T/T vs. T/C + C/C, OR = 2.21, 95% CI = 1.17–4.18, p = 0.016).

Conclusions

Our results provided the first evidence that rs3746444 SNP may influence the susceptibility to UC, and both rs3746444 and rs11614913 SNPs may influence the pathophysiological features of UC.

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Abbreviations

UC:

Ulcerative colitis

miRNA:

MicroRNAs

PCR:

Polymerase chain reaction

RFLP:

Restricted fragment length polymorphism

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Authors and Affiliations

  1. Department of Gastroenterology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan

    Masaaki Okubo, Tomomitsu Tahara, Tomoyuki Shibata, Hiromi Yamashita, Masakatsu Nakamura, Daisuke Yoshioka, Joh Yonemura, Yoshio Kamiya, Takamitsu Ishizuka, Yoshihito Nakagawa, Mitsuo Nagasaka, Masami Iwata & Ichiro Hirata

  2. Department of Gastroenterology, Kanazawa Medical University, 1-1 Daigaku, Uchinadamachi, Ishikawa, 920-0293, Japan

    Hideto Yamada & Tomiyasu Arisawa

Authors
  1. Masaaki Okubo
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  2. Tomomitsu Tahara
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  3. Tomoyuki Shibata
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  4. Hiromi Yamashita
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  12. Masami Iwata
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  13. Hideto Yamada
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  14. Ichiro Hirata
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  15. Tomiyasu Arisawa
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Corresponding author

Correspondence to Tomomitsu Tahara.

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Okubo, M., Tahara, T., Shibata, T. et al. Association Study of Common Genetic Variants in Pre-microRNAs in Patients with Ulcerative Colitis. J Clin Immunol 31, 69–73 (2011). https://doi.org/10.1007/s10875-010-9461-y

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  • DOI: https://doi.org/10.1007/s10875-010-9461-y

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