Abstract
Esophageal cancer is the eighth most common cancer worldwide and fourth most common in developing countries. Altered glycosylation pattern of cell membrane molecules along with inflammation is a characteristic attribute of oncogenesis. Galectin-4, a tandem repeat galectin, has shown effect on cancer progression/metastasis in digestive system cancers. This role of galectin-4 can be attributed to variations in LGALS4, gene encoding galectin-4. The present case–control study was designed to analyze four intronic SNPs in LGALS4 with susceptibility toward esophageal cancer.Esophageal cancer cases and age- and gender-matched apparently healthy individuals were recruited for the present study. Genotyping of rs8113319, rs4802886, rs4802887, and rs12610990 was carried out using Sanger sequencing and PCR–RFLP. MedCalc software, SNPStats and SHEsis online platform were used for statistical analysis.Genotypic analyses revealed an overall increased heterozygosity of rs12610990, rs4802886, and rs4802887, and AA genotype of rs8113319 in the study participants. Haplotypic analyses also revealed a predominance of AAAT haplotype in the cases. Moreover, combined presence of wild alleles of rs4802886 and rs4802887 could influence protection toward disease, and combined presence of wild alleles of rs12610990 and rs8113319 could influence disease susceptibility. Furthermore, a strong linkage disequilibrium was also observed between the SNPs. Further studies are underway to validate galectin-4 and its genetic variants as blood-based biomarkers in early disease diagnosis, improving treatment outcome.
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All data generated or analyzed during this study are included in this article [and its supplementary information file].
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Acknowledgements
We are grateful to the staff of Department of Radiation Oncology, GND Hospital Government Medical College, Amritsar (Pb) and Sri Guru Ramdas Institute of Medical Sciences and Research, Amritsar (Pb) for their invaluable assistance during sample collection. We are also grateful to the study participants for providing their samples for this study. SK would also like to acknowledge Council of Scientific and Industrial Research (CSIR), New Delhi for providing Ph.D. fellowship.
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SK reviewed the literature, was involved in designing as well as, performing experiments, analysis and interpretation of the data obtained and draft the manuscript. RD and JS were instrumental in sample collection and esophageal cancer diagnosis. MK, JS, and NK contributed in the experimental design, data analysis, manuscript editing and supervision. All authors read and approved the final manuscript.
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Kaur, S., Devgan, R., Singh, J. et al. Analysis of Molecular Genetic Variants of Lgals4 in Esophageal Cancer: A Preliminary Report. Biochem Genet (2024). https://doi.org/10.1007/s10528-024-10780-y
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DOI: https://doi.org/10.1007/s10528-024-10780-y