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Statistical medium optimization and biodegradative capacity of Ralstonia eutropha toward p-nitrophenol

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Abstract

The effect of p-nitrophenol (PNP) concentration with or without glucose and yeast extract on the growth and biodegradative capacity of Ralstonia eutropha was examined. The chemical constituents of the culture medium were modeled using a response surface methodology. The experiments were performed according to the central composite design arrangement considering PNP, glucose and yeast extract as the selected variables whose influences on the degradation was evaluated (shaking in reciprocal mode, temperature of 30°C, pH 7 and test time of about 9 h). Quadratic polynomial regression equations were used to quantitatively explain variations between and within the models (responses: the biodegradation capacity and the biomass formation). The coefficient of determination was high (R 2adjusted  = 0.9783), indicating the constructed polynomial model for PNP biodegradative capacity explains the variation between the regressors fairly well. A PNP removal efficiency of 74.5% occurred within 9 h (15 mg/L as the initial concentration of PNP with use of yeast extract at 0.5 g/L).

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Abbreviations

PNP:

p-nitrophenol

LC:

Liquid culture

RSM:

Response surface methodology

CCD:

Central composite design

NAs:

Nitroaromatics

NADPH:

Nicotinamide adenine dinucleotide phosphate

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Authors and Affiliations

  1. Chemical Engineering Department, Amirkabir University of Technology, Tehran, Iran

    Zeinab Salehi, Farzaneh Vahabzadeh & Morteza Sohrabi

  2. Research Center for Biochemical and Environmental Engineering, Toyo University, Kawagoe, Saitama, Japan

    Zeinab Salehi & Hussein Tawfiq Znad

  3. Chemical Engineering Department, University of Tehran, Tehran, Iran

    Shohreh Fatemi

Authors
  1. Zeinab Salehi
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  2. Farzaneh Vahabzadeh
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  3. Morteza Sohrabi
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  4. Shohreh Fatemi
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  5. Hussein Tawfiq Znad
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Correspondence to Farzaneh Vahabzadeh.

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Salehi, Z., Vahabzadeh, F., Sohrabi, M. et al. Statistical medium optimization and biodegradative capacity of Ralstonia eutropha toward p-nitrophenol. Biodegradation 21, 645–657 (2010). https://doi.org/10.1007/s10532-010-9332-5

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  • DOI: https://doi.org/10.1007/s10532-010-9332-5

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