Abstract
Small RNA (RyhB) was overexpressed artificially using an arabinose-inducible system in Escherichia coli and resulted in more succinate (7-fold) accumulation, which suggested that RyhB had a strong effect on sdhCDAB genes. Acetate was also increased indicating that RyhB had a comprehensive influence on glucose central metabolism. RyhB might therefore be useful for metabolic engineering of E. coli.
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Acknowledgments
This research was financially supported by a Grant from the National Natural Science Foundation of China (31070092), a Grant of the National Basic Research Program of China (2011CB707405) and a Grant of Key Scientific and Technological Program of Shandong province (2010GSF10202).
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Kang, Z., Wang, X., Li, Y. et al. Small RNA RyhB as a potential tool used for metabolic engineering in Escherichia coli . Biotechnol Lett 34, 527–531 (2012). https://doi.org/10.1007/s10529-011-0794-2
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DOI: https://doi.org/10.1007/s10529-011-0794-2