Abstract
Autochthonous strains of Saccharomyces cerevisiae from traditional starters used for the production of rice-based ethnic fermented beverage in North East India were examined for their genetic polymorphism using mitochondrial DNA-RFLP and electrophoretic karyotyping. Mitochondrial DNA-RFLP analysis of S. cerevisiae strains with similar technological origins from hamei starter of Manipur and marcha starter of Sikkim revealed widely separated clusters based on their geographical origin. Electrophoretic karyotyping showed high polymorphism amongst the hamei strains within similar mitochondrial DNA-RFLP cluster and one unique karyotype of marcha strain was widely distributed in the Sikkim-Himalayan region. We conceptualized the possibility of separate domestication events for hamei strains in Manipur (located in the Indo-Burma biodiversity hotspot) and marcha strains in Sikkim (located in Himalayan biodiversity hotspot), as a consequence of less homogeneity in the genomic structure between these two groups, their clear separation being based on geographical origin, but not on technological origin and low strain level diversity within each group. The molecular markers developed based on HinfI-mtDNA-RFLP profile and the chromosomal doublets in chromosome VIII position of Sikkim-Himalayan strains could be effectively used as geographical markers for authenticating the above starter strains and differentiating them from other commercial strains.
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This research was supported by the Department of Science and Technology (DST), Govt. of India-sponsored Indo-Italian Programme of Cooperation in Science and Technology 2005–2007 (BS-8).
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10482_2011_9612_MOESM1_ESM.doc
Fig. S1 Location of two geographical regions (Manipur and Sikkim) of North East India selected for studying genetic polymorphism of S. cerevisiae strains with similar technological origin. Grey area Indo-Burma biodiversity hotspot, dark area Himalayan biodiversity hotspot. The Brahmaputra River flow separating Manipur and Sikkim is highlighted with green color line. (DOC 349 kb)
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Jeyaram, K., Tamang, J.P., Capece, A. et al. Geographical markers for Saccharomyces cerevisiae strains with similar technological origins domesticated for rice-based ethnic fermented beverages production in North East India. Antonie van Leeuwenhoek 100, 569–578 (2011). https://doi.org/10.1007/s10482-011-9612-z
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DOI: https://doi.org/10.1007/s10482-011-9612-z