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BMP-2/PLGA Delayed-Release Microspheres Composite Graft, Selection of Bone Particulate Diameters, and Prevention of Aseptic Inflammation for Bone Tissue Engineering

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Abstract

Autogenous bone grafts are widely used in the repair of bone defects. Growth factors such as bone morphogenetic protein 2 (BMP-2) can induce bone regeneration and enhance bone growth. The combination of an autogenous bone graft and BMP-2 may provide a better osteogenic effect than either treatment alone, but BMP-2 is easily inactivated in body fluid. The objective of this study was to develop a technique that can better preserve the in vivo activity of BMP-2 incorporated in bone grafts. In this study, we first prepared BMP-2/poly(lactic-co-glycolic acid) (PLGA) delayed-release microspheres, and then combined collagen, the delayed-release microspheres, and rat autologous bone particulates to form four groups of composite grafts with different combinations: collagen in group A; collagen combined with bone particulates in group B; collagen combined with BMP-2/PLGA delayed-release microspheres in group C; and collagen combined with both bone particulates and BMP-2/PLGA delayed-release microspheres in group D. The four groups of composite grafts were implanted into the gluteus maximus pockets in rats. The ectopic osteogenesis and ALP level in group D (experimental group) were compared with those in groups A, B, and C (control groups) to study whether it had higher osteogenic capability. Results showed that the composite graft design increased the utility of BMP-2 and reduced the required dose of BMP-2 and volume of autologous bone. The selection of bone particulate diameter had an impact on the osteogenetic potential of bone grafts. Collagen prevented the occurrence of aseptic inflammation and improved the osteoinductivity of BMP-2. These results showed that this composite graft design is effective and feasible for use in bone repair.

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Acknowledgment

This study was supported by the National Natural Science Foundation of China (grant no. 30371441).

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, The First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng St., Nangang District, Harbin, Heilongjiang, 150001, China

    Ye Ji, Gong ping Xu, Zhi peng Zhang, Jing jun Xia & Jing long Yan

  2. Central Laboratory, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China

    Shang ha Pan

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  1. Ye Ji
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  2. Gong ping Xu
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  3. Zhi peng Zhang
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  4. Jing jun Xia
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Correspondence to Jing long Yan.

Additional information

Associate Editor Peter E. McHugh oversaw the review of this article.

Ye Ji and Gong ping Xu contributed equally.

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Ji, Y., Xu, G.p., Zhang, Z.p. et al. BMP-2/PLGA Delayed-Release Microspheres Composite Graft, Selection of Bone Particulate Diameters, and Prevention of Aseptic Inflammation for Bone Tissue Engineering. Ann Biomed Eng 38, 632–639 (2010). https://doi.org/10.1007/s10439-009-9888-6

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  • DOI: https://doi.org/10.1007/s10439-009-9888-6

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