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Rapid detection of Panton–Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene

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Abstract

In order to assess the speed and accuracy of a real-time PCR assay targeting the lukS-PV gene of Panton–Valentine leukocidin (PVL)-positive Staphylococcus aureus, 700 S. aureus strains were tested and the results were compared to those achieved with block cycler PCR. Cross-reactivity was tested with 166 other bacterial species. Using this homogeneous real-time PCR assay format, the presence or absence of genetic information for PVL, which is also found in community-associated methicillin-resistant S. aureus, was correctly identified from pure culture and directly in various types of clinical specimens.

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Authors and Affiliations

  1. Institute of Medical Microbiology and Hygiene, University Hospital of Regensburg, Franz-Josef-Strauss-Allee 11, 93053, Regensburg, Germany

    U. Reischl, N. Lehn & H. Linde

  2. Clinical Microbiology, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH, 44195, USA

    M. J. Tuohy, G. S. Hall & G. W. Procop

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  1. U. Reischl
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  2. M. J. Tuohy
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  3. G. S. Hall
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  5. N. Lehn
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  6. H. Linde
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Correspondence to U. Reischl.

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Reischl, U., Tuohy, M.J., Hall, G.S. et al. Rapid detection of Panton–Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene. Eur J Clin Microbiol Infect Dis 26, 131–135 (2007). https://doi.org/10.1007/s10096-007-0254-z

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