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Hepatocyte differentiation from embryonic stem cells and umbilical cord blood cells

  • Published:
Journal of Hepato-Biliary-Pancreatic Surgery

Abstract

With the development of regeneration medicine, many researchers have attempted hepatic differentiation from nonhepatic-origin cell sources. The differentiation of embryonic stem (ES) cells into hepatocyte-like cells has been reported in several papers. Mouse ES cells have shown a potential to develop into hepatocyte-like cells in vitro on the basis of hepatic gene expression after adding several growth factors. We transplanted cultured embryoid body (EB) cells (male) into female mice. A liver specimen of the recipient was examined by immunohistochemical staining for albumin and fluorescence in situ hybridization for the Y chromosome after transplantation. Both Y chromosome- and albumin-positive cells were recognized in the recipient female liver, and were considered to be hepatocyte-like cells derived from ES cells containing the Y chromosome. Many groups, including ourselves, have studied hepatocyte-like cell differentiation from umbilical cord blood cells (UBCs). We cultured nucleated cells isolated from UBCs. Using immunostaining, ALB-positive and CK-19-positive cells were recognized in the culture. Dual staining of ALB and CK-19 demonstrated that ALB was coexpresed with CK-19, suggesting the existence of hepatic progenitors. In this review, we consider recent studies of the differentiation of hepatocytes from nonhepatic origins, especially ES cells and umbilical cord blood.

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Authors and Affiliations

  1. Department of Hepato-Biliary-Pancreatic Surgery, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan

    Kenichi Teramoto, Yuji Kumashiro & Shigeki Arii

  2. Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan

    Kinji Asahina, Keiko Shimizu-Saito & Hirobumi Teraoka

  3. Department of General Medicine, Graduate School of Medicine and Dentistry, Tokyo Medical and Dental University, Tokyo, Japan

    Sei Kakinuma, Ryoko Chinzei & Yujiro Tanaka

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  1. Kenichi Teramoto
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  2. Kinji Asahina
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Teramoto, K., Asahina, K., Kumashiro, Y. et al. Hepatocyte differentiation from embryonic stem cells and umbilical cord blood cells. J Hepatobiliary Pancreat Surg 12, 196–202 (2005). https://doi.org/10.1007/s00534-005-0980-5

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  • DOI: https://doi.org/10.1007/s00534-005-0980-5

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