Abstract
Molecular phylogenetic analysis was carried out for 21 strains of Trypanosoma cruzi, nine of which were obtained from Guatemala and 12 from South America. Phylogenetic trees were constructed using the nucleotide sequences of two nuclear gene regions, dihydrofolate reductase–thymidylate synthase (DHFR-TS) and trypanothione reductase (TR), and contiguous portions of two mitochondrial genes, cytochrome oxidase subunit II (COII) and reduced nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1). Possible genetic exchange between the rather divergent lineages of T. cruzi II from South America was suggested in the trees of the two nuclear genes. T. cruzi I strains obtained from Guatemala and Colombia were identical in all the genes examined, but other T. cruzi I isolates from South America were rather polymorphic in the DHFR-TS and mitochondrial genes. No genetic exchange was identified between T. cruzi I populations from Central and South America in the present study.
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References
Anonymous (1999) Recommendations from a satellite meeting. Mem Inst Oswaldo Cruz 94:429–432
Ayala FJ (1993) Trypanosoma and Leishmania have clonal population structures of epidemiological significance. Biol Res 26:47–63
Barnabe C, Brisse S, Tibayrenc M (2000) Population structure and genetic typing of Trypanosoma cruzi, the agent of Chagas’ disease: a multilocus enzyme electrophoresis approach. Parasitology 120:513–526
Bastrenta B, Bosseno MF, Barnabe C, Tibayrenc M, Breniere SF (1999) Restriction fragment length polymorphism of 195 bp repeated satellite DNA of Trypanosoma cruzi supports the existence of two phylogenetic groups. Mem Inst Oswaldo Cruz 94:323–328
Bogliolo AR, Lauria-Pires L, Gibson WC (1996) Polymorphism in Trypanosoma cruzi: evidence of genetic recombination. Acta Trop 61:31–40
Briones MR, Souto RP, Stolf BS, Zingales B (1999) The evolution of two Trypanosoma cruzi subgroups inferred from rRNA genes can be correlated with the interchange of American mammalian faunas in the Cenozoic and has implications to pathogenicity and host specificity. Mol Biochem Parasitol 104:219–232
Brisse S, Barnabe C, Tibyrenc M (2000a) dentification of six Trypanosoma cruzi phylogenetic lineages by random amplified polymorphic DNA and multilocus enzyme electrophoresis. Int J Parasitol 30:35–44
Brisse S, Dujardin J-C, Tibayrenc M (2000b) Identification of six Trypanosoma cruzi lineages by sequence-characterised amplified region markers. Mol Biochem Parasitol 111:95–105
Brisse S, Verhoef J, Tibayrenc M (2001) haracterization of large and small subunit rRNA and mini-exon genes further supports the distinction of sex Trypanosoma cruzi lineages. Int J Parasitol 31:1218–1226
Brisse S, Henriksson J, Barnabe C, Douzery EJP, Berkvens D, Serrano M, De Carvalho MRC, Buck GA, Dujardin JC, Tibayrenc M (2003) Evidence for genetic exchange and hybridization in Trypanosoma cruzi based on nucleotide sequences and molecular karyotype. Infect Genet Evol 2:173–183
Carrasco HJ, Frame IA, Valente SA, Miles MA (1996) Genetic exchange as a possible source of genomic diversity in sylvatic populations of Trypanosoma cruzi. Am J Trop Med Hyg 54:418–424
DeLeon MP, Yanagi T, Kikuchi M, Mu J, Ayau O, Matta V, Paz M, Juarez S, Kanbara H, Tada I, Hirayama K (1998) Characterization of Trypanosoma cruzi populations by DNA polymorphism of the cruzipain gene detected by single-stranded DNA conformation polymorphism (SSCP) and direct sequencing. Int J Parasitol 28:1867–1874
Gaunt MW, Yeo M, Frame IA, Stothard JR, Carrasco HJ, Taylor MC, Mena SS, Veazey P, Miles GA, Acosta N, de Arias AR, Miles MA (2003) Mechanism of genetic exchange in American trypanosomes. Nature 27:936–939
Higo H, Yanagi T, Matta V, Agatsuma T, Kanbara H, Tada I, Deleon MP, Monroy C, Tabaru Y (1997) Genetic structure of Trypanosoma cruzi in Central America and its comparison with South American strains. Int J Parasitol 27:1369–1374
Higo H, Yanagi T, Matta V, Agatsuma T, Cruz-Reyes A, Uyema N, Monroy C, Kanbara H, Tada I (2000) Genetic structure of Trypanosoma cruzi in American continents: special emphasis on sexual reproduction in Central America. Parasitology 121:403–408
Higo H, Miura S, Horio M, Mimori T, Hamano S, Agatsuma T, Yanagi T, Cruz-Reyes A, Uyema N, Arias AR, Matta V, Akahane H, Hirayama K, Takeuchi T, Tada I, Himeno K (2004) Genotypic variation among lineages of Trypanosoma cruzi and its geographic aspects. Parasitol Int 53:337–344
Higo H, Miura S, Agatsuma T, Mimori T, Yanagi T, Iwagami M, Arias AR, Matta V, Hirayama K, Takeuchi T, Tada I, Himeno K (2007) Identification of Trypanosoma cruzi sublineages by the simple method of single-stranded conformation DNA polymorphism (SSCP). Parasitol Res 100:1023–1031
Kawashita SY, Sanson GF, Fernandes O, Zingales B, Briones MR (2001) Maximum-Likelihood divergence date estimates based on rRNA gene sequences suggest two scenarios of Trypanosoma cruzi intraspecific evolution. Mol Biol Evol 18:2250–2259
Kim KS, Teixeira SM, Kirchhoff LV, Donelson JE (1994) Transcription and editing of cytochrome oxidase II RNAs in Trypanosoma cruzi. J Biol Chem 269:1206–1211
Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245
Lewicka K, Breniere-Campana SF, Barnabe C, Dedet J, Tibayrenc M (1995) An isozyme survey of Trypanosoma cruzi genetic variability in sylvatic cycles from French Guiana. Exp Parasitol 81:20–28
Machado CA, Ayala FJ (2001) Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi. Proc Natl Acad Sci USA 98:7396–7401
Miles MA, Souza A, Povoa M, Shaw JJ, Lainson R, Toye PJ (1978) Isozymic heterogeneity of Trypanosoma cruzi in the first autochthonous patients with Chagas’ disease in Amazonian Brazil. Nature 272:819–821
Miles MA, Lanham SM, De Souza AA, Povoa M (1980) Further enzymic characters of Trypanosoma cruzi and their evaluation for strain identification. Trans R Soc Trop Med Hyg 74:221–237
Momen H (1999) Taxonomy of Trypanosoma cruzi: a commentary on characterization and nomenclature. Mem Inst Oswaldo Cruz 94(Suppl 1):181–184
Reche P, Arrebola R, Olmo A, Santi DV, Gonzalez-Pacanowska D, Ruiz-Perez LM (1994) Cloning and expression of the dihydrofolate reductase-thymidylate synthase gene from Trypanosoma cruzi. Mol Biochem Parasitol 65:247–258
Robello C, Gamarro F, Castanys S, Alvarez-Valin F (2000) Evolutionary relationships in Trypanosoma cruzi: molecular phylogenetics supports the existence of a new major lineage of strains. Gene 246:331–338
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Souto RP, Fernandes O, Macedo AM, Campbell DA, Zingales B (1996) DNA markers define two major phylogenetic lineages of Trypanosoma cruzi. Mol Biochem Parasitol 83:141–152
Sullivan FX, Walsh CT (1991) Cloning, sequencing, overproduction and purification of trypanothione reductase from Trypanosoma cruzi. Mol Biochem Parasitol 44:145–147
Tibayrenc M (1995) Population genetics of parasitic protozoa and other microorganisms. Adv Parasitol 36:47–115
Tibayrenc M, Ayala FJ (1988) Isozyme variability in Trypanosoma cruzi, the agent of Chagas’ disease: genetical, taxonomical, and epidemiological significance. Evolution 42:277–292
Tibayrenc M, Kjellberg F, Ayala FJ (1990) A clonal theory of parasitic protozoa: the population structures of Entamoeba, Giardia, Leishmania, Naegleria, Plasmodium, Trichomonas, and Trypanosoma and their medical and taxonomical consequences. Proc Natl Acad Sci USA 87:2414–2418
Acknowledgments
This study was partly supported by a Grant-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan and the Japan Society of Promotion of Science to T.A. (16659113, 16406010).
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Iwagami, M., Higo, H., Miura, S. et al. Molecular phylogeny of Trypanosoma cruzi from Central America (Guatemala) and a comparison with South American strains. Parasitol Res 102, 129–134 (2007). https://doi.org/10.1007/s00436-007-0739-9
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DOI: https://doi.org/10.1007/s00436-007-0739-9