Abstract
A haplosporidian parasite was detected in the polychaete Syllis nipponica via the amplification and sequencing of the 18S rDNA locus. This provides additional evidence of haplosporidians in polychaetes, the host group from which the first species of the phylum were described about a century ago. In phylogenetic analyses, the parasite was determined to place among mollusk-specific taxa. As such, separate taxonomic groups for haplosporidians infecting different host phyla should not be anticipated. These findings also underscore the as-yet unknown life cycles of commercially important parasites. Attempts to visualize spores of the parasite directly with scanning electron microscopy did not succeed.
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References
Ball SJ, Neville JE (1979) Minchinia chitonis (Lankester, 1885) Labbé, 1896, a haplosporidian parasite of the chiton Lepidochitona cinereus. J Molluscan Stud 45:340–344
Bleidorn C, Vogt L, Bartolomaeus T (2003) New insights into polychaete phylogeny (Annelida) inferred from 18S rDNA sequences. Mol Phylogenet Evol 29:279–288
Borda E, Siddall ME (2004) Arhynchobdellida (Annelida: Oligochaeta: Hirudinida): phylogenetic relationships and evolution. Mol Phylogenet Evol 30:213–225
Brown S, Rouse GW, Hutchings P, Colgan D (1999) Assessing the usefulness of histone H3, U2 snRNA and 28S rDNA in analyses of polychaete relationships. Aust J Zool 47:499–516
Burreson EM, Ford SE (2004) A review of recent information on the Haplosporidia, with special reference to Haplosporidium nelsoni (MSX). Aquat Living Resour 17:499–517
Burreson EM, Stokes NA, Friedman CS (2000) Increased virulence in an introduced pathogen: Haplosporidium nelsoni (MSX) in the eastern oyster Crassostrea virginica. J Aquat Anim Health 12:1–8
Caullery M, Mesnil F (1899) Sur le genre Aplosporidium (nov) et l’ordre nouveau des Aplosporidies. C R Soc Biol (Paris) 51:789–791
Caullery M, Mesnil F (1905) Sur quelques nouvelles haplosporidies d’Anéllide. C R Soc Biol (Paris) 58:580–583
DeSalle R, Egan MG, Siddall M (2005) The unholy trinity: taxonomy, species delimitation and DNA barcoding. Phil Trans R Soc Lond B 360:1905–1916
Farris JS, Albert VA, Källersjö M, Lipscomb D, Kluge AG (1996) Parsimony jackknifing outperforms neighbor-joining. Cladistics 12:99–124
Hillman RE, Maciolek NJ, Lahey JI, Belmore CI (1982) Effects of a haplosporidian parasite, Haplosporidium sp., on species of the molluscan woodborer Teredo in Barnegat Bay, New Jersey. J Invert Pathol 40:307–319
Siddall ME, Borda E, Rouse GW (2004) Toward a tree of life for Annelida. In: Cracraft J, Donoghue MJ (eds) Assembling the tree of life. Oxford Univ. Press, Oxford, UK, pp 237–251
Stokes NA, Siddall ME, Burreson EM (1995a) Detection of Haplosporidium nelsoni (Haplosporidia: Haplosporidiidae) in oysters by PCR amplification. Dis Aquat Organ 23:145–152
Stokes NA, Siddall ME, Burreson EM (1995b) Small subunit ribosomal RNA gene sequence of Minchinia teredinis (Haplosporidia: Haplosporidiidae) and a specific DNA probe for its detection. J Invertebr Pathol 65:300–308
Swofford DL (2002) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. Sinauer, Sunderland, Massachusetts
Acknowledgements
We thank Eijiroh Nishi for providing us with specimens of S. nipponica. This work was supported by the AMNH Lerner Grey fund for marine research and by a grant from the Richard Lounsbery Foundation.
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Siddall, M.E., Aguado, MT. Molecular phylogenetic evidence of a haplosporidian parasite infecting the polychaete Syllis nipponica (Imajima, 1966). Parasitol Res 99, 309–312 (2006). https://doi.org/10.1007/s00436-006-0171-6
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DOI: https://doi.org/10.1007/s00436-006-0171-6